Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 07 2022
Historique:
received: 17 02 2022
accepted: 25 07 2022
entrez: 29 7 2022
pubmed: 30 7 2022
medline: 3 8 2022
Statut: epublish

Résumé

Cyclophosphamide (CYP) damages all mucosal defence lines and induces hemorrhagic cystitis (HC) leading to detrusor overactivity. Patients who undergo combined chemio-radiotherapy are at higher risk of HC. Potentilla chinensis extract (PCE) prevent oxidative stress-dependent diseases. Thus, the aim of the study was to investigate the effect of PCE on urinary bladder function in CYP-induced HC in preclinical study. 60 rats were divided into 4 groups, as follows: I-control, II-rats with CYP-induced HC, III-rats received PCE in dose of 500 mg/kg, and IV-rats with CYP-induced HC which received PCE in dose of 500 mg/kg. PCE or vehicle were administered orally for 14 days. The cystometry was performed 3 days after the last dose of the PCE. Next, urothelium thickness and oedema measurement and biochemical analyses were performed. Cyclophosphamide induced hemorrhagic cystitis. PCE had no influence on the urinary bladder function and micturition cycles in normal rats. PCE diminished the severity of CYP-induced hemorrhagic cystitis. In the urothelium the cyclophosphamide induced the elevation of CGRP, TNF-α, IL-6, IL-1β, OTC

Identifiants

pubmed: 35906474
doi: 10.1038/s41598-022-17393-8
pii: 10.1038/s41598-022-17393-8
pmc: PMC9338276
doi:

Substances chimiques

Cyclophosphamide 8N3DW7272P

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

13076

Informations de copyright

© 2022. The Author(s).

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Auteurs

Kajetan Juszczak (K)

Department of Urology and Andrology, Collegium Medicum, Nicolaus Copernicus University, M. Curie Skłodowskiej 9, 85-094, Bydgoszcz, Poland. kaj.juszczak@gmail.com.

Jan Adamowicz (J)

Department of Regenerative Medicine, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.

Łukasz Zapała (Ł)

Clinic of General, Oncological and Functional Urology, Medical University of Warsaw, Warsaw, Poland.

Tomasz Kluz (T)

Department of Gynecology and Obstetrics, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland.

Przemysław Adamczyk (P)

Department of General and Oncological Urology, Nicolaus Copernicus Hospital, Torun, Poland.

Artur Wdowiak (A)

Chair of Obstetrics and Gynecology, Faculty of Health Sciences, Medical University of Lublin, Lublin, Poland.

Iwona Bojar (I)

Department of Women's Health, Institute of Rural Health in Lublin, Lublin, Poland.

Marcin Misiek (M)

Department of Gynecologic Oncology, Holy Cross Cancer Center, Kielce, Poland.

Magdalena Emilia Grzybowska (ME)

Department of GynecologyGynecological Oncology and Gynecological Endocrinology, Medical, University of Gdańsk, Gdańsk, Poland.

Klaudia Stangel-Wójcikiewicz (K)

Department of Gynecology and Oncology, Jagiellonian University Medical College, Cracow, Poland.

Ewa Poleszak (E)

Chair and Department of Applied and Social Pharmacy, Laboratory of Preclinical Testing, Medical University of Lublin, Lublin, Poland.

Marta Pokrywczyńska (M)

Department of Regenerative Medicine, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.

Tomasz Drewa (T)

Department of Urology and Andrology, Collegium Medicum, Nicolaus Copernicus University, M. Curie Skłodowskiej 9, 85-094, Bydgoszcz, Poland.

Andrzej Wróbel (A)

Second Department of Gynaecology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland. wrobelandrzej@yahoo.com.

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Classifications MeSH