Interpretation of cerebrospinal fluid analysis from recumbent cows using different thresholds of red blood cell count.
CSF analysis
cattle
neurology
nonambulatory
recumbent
ruminant
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
22
07
2021
accepted:
15
07
2022
pubmed:
31
7
2022
medline:
28
9
2022
entrez:
30
7
2022
Statut:
ppublish
Résumé
Hemodilution of the cerebrospinal fluid (CSF) could confound interpretation of results. Accurately predicting total nucleated cells count (TNCC) and total protein concentration (TPC) attributable to hemodilution is difficult. To determine the effects of hemodilution on TPC and TNCC in bovine CSF. Retrospective review of CSF analysis results of downer dairy cows treated at Centre hospitalier universitaire vétérinaire between January 2006 and December 2014. Descriptive statistics were performed using 3 scenarios. Among the 235 samples included, red blood cell (RBC) count (RBCC) ranged from 0 to 869 220 RBC/μL (median = 6.6), TPC ranged from 0.04 to 6.51 g/L (median = 0.27), and TNCC ranged from 0 to 7500 cell/μL (median = 1.1). Among the 157 samples that had <30 RBC/μL (a threshold used in other species), TPC and TNCC varied between 0.13 and 1.06 g/L (median = 0.27) and between 0 and 31.4 cell/μL (median = 0.6), respectively. Eighty-four samples had TPC <0.25 g/L and TNCC ≤4.5 cell/μL. Among those 84 samples, RBCC varied between 0 and 1290 RBC/μL (median = 4.7). In 20 samples, TNCC was 0 with a variation in RBCC between 0 and 840 RBC/μL (median = 3.9). No strong correlations between RBCC and TNCC and TPC were found. A cutoff around 200 RBC/μL is proposed as clinically meaninful in bovine CSF. Results between 200 and 1290 RBC/μL are equivocal.
Sections du résumé
BACKGROUND
BACKGROUND
Hemodilution of the cerebrospinal fluid (CSF) could confound interpretation of results. Accurately predicting total nucleated cells count (TNCC) and total protein concentration (TPC) attributable to hemodilution is difficult.
OBJECTIVE
OBJECTIVE
To determine the effects of hemodilution on TPC and TNCC in bovine CSF.
METHODS
METHODS
Retrospective review of CSF analysis results of downer dairy cows treated at Centre hospitalier universitaire vétérinaire between January 2006 and December 2014. Descriptive statistics were performed using 3 scenarios.
RESULTS
RESULTS
Among the 235 samples included, red blood cell (RBC) count (RBCC) ranged from 0 to 869 220 RBC/μL (median = 6.6), TPC ranged from 0.04 to 6.51 g/L (median = 0.27), and TNCC ranged from 0 to 7500 cell/μL (median = 1.1). Among the 157 samples that had <30 RBC/μL (a threshold used in other species), TPC and TNCC varied between 0.13 and 1.06 g/L (median = 0.27) and between 0 and 31.4 cell/μL (median = 0.6), respectively. Eighty-four samples had TPC <0.25 g/L and TNCC ≤4.5 cell/μL. Among those 84 samples, RBCC varied between 0 and 1290 RBC/μL (median = 4.7). In 20 samples, TNCC was 0 with a variation in RBCC between 0 and 840 RBC/μL (median = 3.9). No strong correlations between RBCC and TNCC and TPC were found.
CONCLUSIONS
CONCLUSIONS
A cutoff around 200 RBC/μL is proposed as clinically meaninful in bovine CSF. Results between 200 and 1290 RBC/μL are equivocal.
Identifiants
pubmed: 35906868
doi: 10.1111/jvim.16502
pmc: PMC9511068
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1837-1842Informations de copyright
© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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