In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
09
03
2022
revised:
13
06
2022
accepted:
11
07
2022
pubmed:
31
7
2022
medline:
10
8
2022
entrez:
30
7
2022
Statut:
ppublish
Résumé
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
Identifiants
pubmed: 35907349
pii: S1873-5061(22)00219-7
doi: 10.1016/j.scr.2022.102870
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102870Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.