Identification of Daboia siamensis venome using integrated multi-omics data.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 07 2022
30 07 2022
Historique:
received:
10
02
2022
accepted:
22
07
2022
entrez:
30
7
2022
pubmed:
31
7
2022
medline:
3
8
2022
Statut:
epublish
Résumé
Snakebite, classified by World Health Organization as a neglected tropical disease, causes more than 100,000 deaths and 2 million injuries per year. Currently, available antivenoms do not bind with strong specificity to target toxins, which means that severe complications can still occur despite treatment. Moreover, the cost of antivenom is expensive. Knowledge of venom compositions is fundamental for producing a specific antivenom that has high effectiveness, low side effects, and ease of manufacture. With advances in mass spectrometry techniques, venom proteomes can now be analyzed in great depth at high efficiency. However, these techniques require genomic and transcriptomic data for interpreting mass spectrometry data. This study aims to establish and incorporate genomics, transcriptomics, and proteomics data to study venomics of a venomous snake, Daboia siamensis. Multiple proteins that have not been reported as venom components of this snake such as hyaluronidase-1, phospholipase B, and waprin were discovered. Thus, multi-omics data are advantageous for venomics studies. These findings will be valuable not only for antivenom production but also for the development of novel therapeutics.
Identifiants
pubmed: 35907887
doi: 10.1038/s41598-022-17300-1
pii: 10.1038/s41598-022-17300-1
pmc: PMC9338987
doi:
Substances chimiques
Antivenins
0
Proteome
0
Venoms
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13140Informations de copyright
© 2022. The Author(s).
Références
Genome Res. 2017 May;27(5):757-767
pubmed: 28381613
Nucleic Acids Res. 2018 Mar 16;46(5):2699
pubmed: 29425356
BMC Bioinformatics. 2011 Dec 22;12:491
pubmed: 22192575
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20651-6
pubmed: 24297900
Curr Biol. 2016 Sep 26;26(18):2434-2445
pubmed: 27641771
PLoS Negl Trop Dis. 2017 Feb 3;11(2):e0005361
pubmed: 28158193
Bull World Health Organ. 1998;76(5):515-24
pubmed: 9868843
Lancet. 2000 Dec 16;356(9247):2104
pubmed: 11145528
Methods Mol Biol. 2019;1962:227-245
pubmed: 31020564
Genome Biol Evol. 2017 Oct 1;9(10):2640-2649
pubmed: 29048530
Nat Genet. 2020 Jan;52(1):106-117
pubmed: 31907489
J Emerg Trauma Shock. 2008 Jul;1(2):97-105
pubmed: 19561988
J Biol Chem. 2009 Apr 10;284(15):9885-91
pubmed: 19208624
Toxicon. 2018 Jan;141:18-24
pubmed: 29170055
J Proteome Res. 2017 Feb 3;16(2):583-598
pubmed: 27936776
Nat Rev Dis Primers. 2017 Sep 14;3:17063
pubmed: 28905944
J Venom Anim Toxins Incl Trop Dis. 2020 Jan 31;26:e20190048
pubmed: 32082369
Toxicon X. 2020 Jul 21;7:100052
pubmed: 32776002
Trop Med Infect Dis. 2018 Jun 15;3(2):
pubmed: 30274462
J Proteomics. 2009 Mar 6;72(2):256-69
pubmed: 19457351
Biochem Soc Trans. 2020 Apr 29;48(2):537-546
pubmed: 32196542
J Evol Biol. 2015 Jul;28(7):1309-20
pubmed: 26012745
Toxicon. 2018 May;146:31-41
pubmed: 29567103
Front Bioeng Biotechnol. 2020 Jul 10;8:703
pubmed: 32766215
Gigascience. 2012 Dec 27;1(1):18
pubmed: 23587118
Appl Microbiol Biotechnol. 2016 Jul;100(14):6165-6181
pubmed: 27245678
Toxins (Basel). 2016 Dec 01;8(12):
pubmed: 27916957
Proc Biol Sci. 2014 Sep 22;281(1791):20141122
pubmed: 25080342
Toxicon. 1987;25(5):547-54
pubmed: 3617089
Toxins (Basel). 2019 Sep 25;11(10):
pubmed: 31557973
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6692-6
pubmed: 3462719
Toxicon. 2000 Nov;38(11):1613-20
pubmed: 10775761
Sci Rep. 2018 Jun 4;8(1):8545
pubmed: 29867131
Toxicon. 2011 Apr;57(5):627-45
pubmed: 21277886
Mol Biol Evol. 2014 Nov;31(11):2836-46
pubmed: 25135948
Int J Mol Sci. 2021 Dec 15;22(24):
pubmed: 34948283
Toxicon. 2015 Dec 1;107(Pt B):266-81
pubmed: 26160495
J Proteomics. 2016 Jul 20;144:73-86
pubmed: 27265321
Toxicon. 2013 Feb;62:75-82
pubmed: 23010165
Brain Behav Evol. 2015 Sep;86(1):58-74
pubmed: 26397110
Mitochondrial DNA. 2013 Apr;24(2):94-6
pubmed: 22994371
Toxicon. 1991;29(11):1279-303
pubmed: 1814005
J Proteomics. 2021 Mar 15;234:104084
pubmed: 33359941
Mol Cell Proteomics. 2019 Dec;18(12):2478-2491
pubmed: 31591261
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20645-50
pubmed: 24297902
Anal Chem. 2005 Oct 15;77(20):6630-9
pubmed: 16223250
J Proteomics. 2015 Oct 14;128:403-23
pubmed: 26342672
Sci Rep. 2018 Jul 26;8(1):11300
pubmed: 30050104
Proc Natl Acad Sci U S A. 2020 Oct 20;117(42):26307-26317
pubmed: 33020272
Gigascience. 2015 Oct 19;4:48
pubmed: 26500767
Genome Biol Evol. 2009 Jul 23;1:205-20
pubmed: 20333191