Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.
Avian influenza
H5 subtype
Rapid
Reverse transcription recombinase-aided amplification assay
Sensitive
Single temperature
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
30 07 2022
30 07 2022
Historique:
received:
07
12
2021
accepted:
06
05
2022
entrez:
30
7
2022
pubmed:
31
7
2022
medline:
3
8
2022
Statut:
epublish
Résumé
The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 10 These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
Sections du résumé
BACKGROUND
The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.
METHODS
In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min.
RESULTS
The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 10
CONCLUSIONS
These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
Identifiants
pubmed: 35907986
doi: 10.1186/s12985-022-01807-0
pii: 10.1186/s12985-022-01807-0
pmc: PMC9338541
doi:
Substances chimiques
Recombinases
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
129Informations de copyright
© 2022. The Author(s).
Références
Arch Virol. 2019 Jul;164(7):1843-1850
pubmed: 31053978
Arch Virol. 2018 Jun;163(6):1455-1461
pubmed: 29429036
Biochem Biophys Res Commun. 2003 Jan 10;300(2):507-15
pubmed: 12504113
Nat Microbiol. 2019 Nov;4(11):1781-1789
pubmed: 31332385
Diagn Microbiol Infect Dis. 2018 Feb;90(2):90-95
pubmed: 29141771
Avian Dis. 1998 Jul-Sep;42(3):517-22
pubmed: 9777152
J Clin Microbiol. 2002 Sep;40(9):3256-60
pubmed: 12202562
Mol Biotechnol. 2010 Jan;44(1):41-50
pubmed: 19757212
J Clin Microbiol. 2009 Feb;47(2):327-34
pubmed: 19052173
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Vet Immunol Immunopathol. 2007 May 15;117(1-2):17-25
pubmed: 17337303
Talanta. 2009 Jul 15;79(2):159-64
pubmed: 19559858
Springerplus. 2016 Dec 1;5(1):2054
pubmed: 27995031
Nihon Rinsho. 1997 Oct;55(10):2542-6
pubmed: 9360369
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2018 Jun 13;30(3):273-277
pubmed: 30019553
Methods Mol Biol. 2007;402:35-60
pubmed: 17951789
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
J Clin Virol. 2015 Aug;69:16-21
pubmed: 26209370
BMC Infect Dis. 2019 Mar 5;19(1):229
pubmed: 30836947
Clin Microbiol Rev. 2007 Apr;20(2):243-67
pubmed: 17428885
Lab Chip. 2010 Apr 7;10(7):887-93
pubmed: 20300675
Nucleic Acids Res. 1992 Apr 11;20(7):1691-6
pubmed: 1579461