Lipoplexes for effective in vitro delivery of microRNAs to adult human cardiac fibroblasts for perspective direct cardiac cell reprogramming.

Cellular Reprogramming Fibroblasts Humans Liposomes MicroRNAs / genetics Phosphates Phosphatidylethanolamines RNA, Messenger Transfection
Adult human cardiac fibroblasts Direct reprogramming Lipoplexes miRcombo microRNAs

Journal

Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142

Informations de publication

Date de publication:
09 2022
Historique:
received: 12 01 2022
revised: 23 06 2022
accepted: 18 07 2022
pubmed: 1 8 2022
medline: 24 9 2022
entrez: 31 7 2022
Statut: ppublish

Résumé

Design of nanocarriers for efficient miRNA delivery can significantly improve miRNA-based therapies. Lipoplexes based on helper lipid, dioleoyl phosphatidylethanolamine (DOPE) and cationic lipid [2-(2,3-didodecyloxypropyl)-hydroxyethyl] ammonium bromide (DE) were formulated to efficiently deliver miR-1 or a combination of four microRNAs (miRcombo) to adult human cardiac fibroblasts (AHCFs). Lipoplexes with amino-to-phosphate groups ratio of 3 (N/P 3) showed nanometric hydrodynamic size (372 nm), positive Z-potential (40 mV) and high stability under storage conditions. Compared to commercial DharmaFECT1 (DF), DE-DOPE/miRNA lipoplexes showed superior miRNA loading efficiency (99 % vs. 64 %), and faster miRNA release (99 % vs. 82 % at 48 h). DE-DOPE/miR-1 lipoplexes showed superior viability (80-100 % vs. 50 %) in AHCFs, a 2-fold higher miR-1 expression and Twinfilin-1 (TWF-1) mRNA downregulation. DE-DOPE/miRcombo lipoplexes significantly enhanced AHCFs reprogramming into induced cardiomyocytes (iCMs), as shown by increased expression of CM markers compared to DF/miRcombo.

Identifiants

DOI: 10.1016/j.nano.2022.102589 PMID: 35908737
pubmed: 35908737
pii: S1549-9634(22)00075-2
doi: 10.1016/j.nano.2022.102589
pii:
doi:

Substances chimiques

Liposomes 0
MicroRNAs 0
Phosphates 0
Phosphatidylethanolamines 0
RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102589

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest There are no conflicts to declare.

Auteurs

Letizia Nicoletti (L)

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.

Camilla Paoletti (C)

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.

Giulia Tarricone (G)

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.

Ilaria Andreana (I)

Department of Drug Science and Technology, University of Turin, Via Pietro Giuria, 11, 10125, Turin, Italy.

Barbara Stella (B)

Department of Drug Science and Technology, University of Turin, Via Pietro Giuria, 11, 10125, Turin, Italy.

Silvia Arpicco (S)

Department of Drug Science and Technology, University of Turin, Via Pietro Giuria, 11, 10125, Turin, Italy.

Carla Divieto (C)

Istituto Nazionale di Ricerca Metrologica, Division of Advanced Materials and Life Sciences, Str. delle Cacce, 91, 10135 Turin, Italy.

Clara Mattu (C)

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.

Valeria Chiono (V)

Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy. Electronic address: valeria.chiono@polito.it.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du phosphore › Acides du phosphore › Acides phosphoriques › Phosphates › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Glycérophosphate › Acides phosphatidiques › Glycérophospholipides › Phosphatidyléthanolamine › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Lipoplexes for effective in vitro delivery of...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Transfection › Lipoplexes for effective in vitro delivery of...