Aedes koreicus, a vector on the rise: Pan-European genetic patterns, mitochondrial and draft genome sequencing.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
18
12
2021
accepted:
27
05
2022
entrez:
1
8
2022
pubmed:
2
8
2022
medline:
4
8
2022
Statut:
epublish
Résumé
The mosquito Aedes koreicus (Edwards, 1917) is a recent invader on the European continent that was introduced to several new places since its first detection in 2008. Compared to other exotic Aedes mosquitoes with public health significance that invaded Europe during the last decades, this species' biology, behavior, and dispersal patterns were poorly investigated to date. To understand the species' population relationships and dispersal patterns within Europe, a fragment of the cytochrome oxidase I (COI or COX1) gene was sequenced from 130 mosquitoes, collected from five countries where the species has been introduced and/or established. Oxford Nanopore and Illumina sequencing techniques were combined to generate the first complete nuclear and mitochondrial genomic sequences of Ae. koreicus from the European region. The complete genome of Ae. koreicus is 879 Mb. COI haplotype analyses identified five major groups (altogether 31 different haplotypes) and revealed a large-scale dispersal pattern between European Ae. koreicus populations. Continuous admixture of populations from Belgium, Italy, and Hungary was highlighted, additionally, haplotype diversity and clustering indicate a separation of German sequences from other populations, pointing to an independent introduction of Ae. koreicus to Europe. Finally, a genetic expansion signal was identified, suggesting the species might be present in more locations than currently detected. Our results highlight the importance of genetic research of invasive mosquitoes to understand general dispersal patterns, reveal main dispersal routes and form the baseline of future mitigation actions. The first complete genomic sequence also provides a significant leap in the general understanding of this species, opening the possibility for future genome-related studies, such as the detection of 'Single Nucleotide Polymorphism' markers. Considering its public health importance, it is crucial to further investigate the species' population genetic dynamic, including a larger sampling and additional genomic markers.
Sections du résumé
BACKGROUND
The mosquito Aedes koreicus (Edwards, 1917) is a recent invader on the European continent that was introduced to several new places since its first detection in 2008. Compared to other exotic Aedes mosquitoes with public health significance that invaded Europe during the last decades, this species' biology, behavior, and dispersal patterns were poorly investigated to date.
METHODOLOGY/PRINCIPAL FINDINGS
To understand the species' population relationships and dispersal patterns within Europe, a fragment of the cytochrome oxidase I (COI or COX1) gene was sequenced from 130 mosquitoes, collected from five countries where the species has been introduced and/or established. Oxford Nanopore and Illumina sequencing techniques were combined to generate the first complete nuclear and mitochondrial genomic sequences of Ae. koreicus from the European region. The complete genome of Ae. koreicus is 879 Mb. COI haplotype analyses identified five major groups (altogether 31 different haplotypes) and revealed a large-scale dispersal pattern between European Ae. koreicus populations. Continuous admixture of populations from Belgium, Italy, and Hungary was highlighted, additionally, haplotype diversity and clustering indicate a separation of German sequences from other populations, pointing to an independent introduction of Ae. koreicus to Europe. Finally, a genetic expansion signal was identified, suggesting the species might be present in more locations than currently detected.
CONCLUSIONS/SIGNIFICANCE
Our results highlight the importance of genetic research of invasive mosquitoes to understand general dispersal patterns, reveal main dispersal routes and form the baseline of future mitigation actions. The first complete genomic sequence also provides a significant leap in the general understanding of this species, opening the possibility for future genome-related studies, such as the detection of 'Single Nucleotide Polymorphism' markers. Considering its public health importance, it is crucial to further investigate the species' population genetic dynamic, including a larger sampling and additional genomic markers.
Identifiants
pubmed: 35913994
doi: 10.1371/journal.pone.0269880
pii: PONE-D-21-39717
pmc: PMC9342712
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0269880Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Annu Rev Entomol. 2014;59:31-49
pubmed: 24397520
Mol Biol Evol. 2017 Dec 1;34(12):3299-3302
pubmed: 29029172
Insect Mol Biol. 1996 Aug;5(3):153-65
pubmed: 8799733
Pathog Glob Health. 2018 May;112(3):107-114
pubmed: 29737236
Parasit Vectors. 2015 Dec 01;8:614
pubmed: 26626019
Parasitol Res. 2018 Oct;117(10):3355-3360
pubmed: 30196322
J Clin Virol. 2018 Oct;107:38-47
pubmed: 30176404
Parasitol Res. 2017 Aug;116(8):2355-2358
pubmed: 28624875
BMC Evol Biol. 2007 Nov 08;7:214
pubmed: 17996036
Parasit Vectors. 2021 Mar 25;14(1):179
pubmed: 33766104
J Med Entomol. 2012 Nov;49(6):1226-32
pubmed: 23270149
Parasit Vectors. 2019 Jul 5;12(1):334
pubmed: 31277680
Parasit Vectors. 2019 Nov 6;12(1):524
pubmed: 31694685
Syst Biol. 2018 Sep 1;67(5):901-904
pubmed: 29718447
PLoS Negl Trop Dis. 2020 Jun 23;14(6):e0008433
pubmed: 32574163
Med Parazitol (Mosk). 2014 Jan-Mar;(1):16-9
pubmed: 24738221
Parasit Vectors. 2018 Dec 17;11(1):662
pubmed: 30558660
PLoS One. 2020 Oct 29;15(10):e0241235
pubmed: 33119650
Parasitol Res. 2017 Dec;116(12):3253-3263
pubmed: 29032497
Int J Environ Res Public Health. 2020 Apr 15;17(8):
pubmed: 32326530
Genome Res. 2017 May;27(5):722-736
pubmed: 28298431
Parasitol Res. 2016 Dec;115(12):4687-4689
pubmed: 27511369
Parasit Vectors. 2015 Mar 23;8:177
pubmed: 25884876
Data Brief. 2021 Apr 10;36:107047
pubmed: 33997197
Genome Biol Evol. 2021 Aug 3;13(8):
pubmed: 34152413
J Arthropod Borne Dis. 2020 Sep 30;14(3):270-276
pubmed: 33644240
Methods Mol Biol. 2019;1962:227-245
pubmed: 31020564
Parasit Vectors. 2016 Feb 03;9:63
pubmed: 26842546
Parasit Vectors. 2021 Oct 14;14(1):534
pubmed: 34649599
Microb Genom. 2017 Sep 14;3(10):e000132
pubmed: 29177090
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
Vector Borne Zoonotic Dis. 2016 Jan;16(1):58-60
pubmed: 26741323
Mol Ecol. 2019 May;28(9):2360-2377
pubmed: 30849200
Parasit Vectors. 2015 Jul 30;8:402
pubmed: 26223377
PLoS One. 2014 Nov 19;9(11):e112963
pubmed: 25409509
Viruses. 2019 Nov 14;11(11):
pubmed: 31739553
PLoS Negl Trop Dis. 2020 Sep 30;14(9):e0008657
pubmed: 32997656
Parasite. 2021;28:52
pubmed: 34142954
Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
pubmed: 7881515
Parasitol Res. 2019 Mar;118(3):1073-1076
pubmed: 30734861
Parasitol Res. 2016 Mar;115(3):1331-4
pubmed: 26614356
Parasit Vectors. 2022 Jun 13;15(1):206
pubmed: 35698108
Mol Ecol Resour. 2015 Mar;15(2):449-57
pubmed: 25143182
Parasit Vectors. 2011 Sep 28;4:188
pubmed: 21951867