Induction of potassium channel regulator KCNE4 in a submandibular lymph node metastasis model.
Animals
Antigens, Differentiation
/ metabolism
Carrier Proteins
/ metabolism
Endothelial Cells
/ metabolism
Humans
Lymph Nodes
/ pathology
Lymphatic Metastasis
/ pathology
Melanoma, Experimental
/ pathology
Mice
Microfilament Proteins
/ metabolism
Potassium Channels
/ metabolism
Potassium Channels, Voltage-Gated
/ metabolism
Sentinel Lymph Node Biopsy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 08 2022
01 08 2022
Historique:
received:
16
03
2022
accepted:
01
07
2022
entrez:
1
8
2022
pubmed:
2
8
2022
medline:
4
8
2022
Statut:
epublish
Résumé
Cancer cells often metastasize to the lymph nodes (LNs) before disseminating throughout the body. Clinically, LN metastasis correlates with poor prognosis and influences treatment options. Many studies have shown that cancer cells communicate with immune and stromal cells to prepare a suitable niche for metastasis. In this study, mice were injected with B16-F10 murine melanoma cells to generate a tongue submandibular lymph node (SLN) metastasis model in which genes of interest could be investigated. Microarray analyses were performed on SLNs, identifying 162 upregulated genes, some of which are known metastasis genes. Among these upregulated genes, Kcne4, Slc7a11, Fscn1, and Gadd45b were not associated with metastasis, and increased expression of Kcne4 and Slc7a11 was confirmed by real-time PCR and immunohistochemistry. The roles of KCNE4 in chemokine production and cell adhesion were examined using primary lymphatic endothelial cells, and demonstrated that Ccl17 and Ccl19, which are involved in melanoma metastasis, were upregulated by KCNE4, as well as Mmp3 matrix metalloproteinase. Expression of KCNE4 was detected in human LNs with metastatic melanoma. In conclusion, we found that LN metastatic melanoma induces KCNE4 expression in the endothelium of LNs.
Identifiants
pubmed: 35915077
doi: 10.1038/s41598-022-15926-9
pii: 10.1038/s41598-022-15926-9
pmc: PMC9343410
doi:
Substances chimiques
Antigens, Differentiation
0
Carrier Proteins
0
FSCN1 protein, human
0
Gadd45b protein, mouse
0
KCNE4 protein, human
0
KCNE4 protein, mouse
0
Microfilament Proteins
0
Potassium Channels
0
Potassium Channels, Voltage-Gated
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
13208Informations de copyright
© 2022. The Author(s).
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