Pulpal expression of erythropoietin and erythropoietin receptor after direct pulp capping in rat.
Alkaline Phosphatase
/ metabolism
Aluminum Compounds
/ pharmacology
Animals
Calcium Hydroxide
/ pharmacology
Dental Pulp
Dental Pulp Capping
Drug Combinations
Erythropoietin
/ metabolism
Inflammation
/ pathology
Oxides
/ pharmacology
Rats
Rats, Wistar
Receptors, Erythropoietin
/ metabolism
Silicates
/ pharmacology
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
/ metabolism
biomaterials
calcium hydroxide
dental pulp exposure
endodontic
inflammation
Journal
European journal of oral sciences
ISSN: 1600-0722
Titre abrégé: Eur J Oral Sci
Pays: England
ID NLM: 9504563
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
22
03
2022
accepted:
17
07
2022
pubmed:
3
8
2022
medline:
23
9
2022
entrez:
2
8
2022
Statut:
ppublish
Résumé
This study aimed to evaluate the effect of direct pulp capping on the expression of erythropoietin (Epo) and Epo-receptor (Epor) genes in relation to the expression of inflammatory and osteogenic genes in rat pulp. Dental pulps of the first maxillary molars of Wistar Albino rats were exposed and capped with either calcium hydroxide or mineral trioxide aggregate, or were left untreated. After 4 wk, animals were euthanized, and maxillae were prepared for histological and real-time polymerase chain reaction analysis. Histological scores of pulp inflammation and mineralization, and relative expressions of Epo, Epor, inflammatory cytokines, and pulp osteogenic genes were evaluated. The capped pulps showed higher expressions of Epo, while the untreated pulps had the highest expression of Epor. Both calcium hydroxide and mineral trioxide aggregate downregulated the expression of tumor necrosis factor alpha compared to untreated controls, and upregulated transforming growth factor beta compared to healthy controls. Alkaline phosphatase expression was significantly higher in experimental groups. Relative expression of Epo negatively correlated with pulp inflammation, and positively correlated with pulp mineralization. Pulp exposure promoted expression of Epor and pro-inflammatory cytokines, while pulp capping promoted expression of Epo, alkaline phosphatase, and downregulated Epor and pro-inflammatory cytokines.
Substances chimiques
Aluminum Compounds
0
Drug Combinations
0
Oxides
0
Receptors, Erythropoietin
0
Silicates
0
Transforming Growth Factor beta
0
Tumor Necrosis Factor-alpha
0
erythropoietin, rat
0
Erythropoietin
11096-26-7
Alkaline Phosphatase
EC 3.1.3.1
Calcium Hydroxide
PF5DZW74VN
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12888Informations de copyright
© 2022 Scandinavian Division of the International Association for Dental Research. Published by John Wiley & Sons Ltd.
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