The Japanese quail chorioallantoic membrane as a model to study an amphiphilic gradient copoly(2-oxazoline)s- based drug delivery system for photodynamic diagnosis and therapy research.


Journal

Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123

Informations de publication

Date de publication:
Dec 2022
Historique:
received: 08 06 2022
accepted: 29 07 2022
pubmed: 3 8 2022
medline: 15 12 2022
entrez: 2 8 2022
Statut: ppublish

Résumé

Amphiphilic gradient copoly(2-oxazoline)s are widely researched in the field of drug delivery. They could be used as a transport system for hydrophobic drugs such as hypericin (HYP). We prepared six gradient copolymers (EtOx)-grad-(ROPhOx) by living cationic ring-opening polymerization of a hydrophilic comonomer 2-ethyl-2-oxazoline (EtOx) and a hydrophobic comonomer 2-(4-alkyloxyphenyl)-2-oxazoline (ROPhOx), with different composition ratio (88:12 and 85:15) and three different alkyl chain lengths of alkyl (R) substituents. As an experimental model, Japanese quail chorioallantoic membrane (CAM) was used. The effect of nanoparticles loaded with HYP was evaluated by the changes of fluorescence intensity during photodynamic diagnosis (PDD) monitored under 405 nm LED light before administration, and 0,1,3 and 24 h after topical administration. The effectiveness of photodynamic therapy (PDT) (405 nm, 285 mW/cm

Identifiants

pubmed: 35917905
pii: S1572-1000(22)00332-5
doi: 10.1016/j.pdpdt.2022.103046
pii:
doi:

Substances chimiques

hypericin 7V2F1075HD
Perylene 5QD5427UN7
Photosensitizing Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103046

Informations de copyright

Copyright © 2022. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of Competing Interest None to declare.

Auteurs

Barbora Kundeková (B)

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84005, Slovakia.

Mariana Máčajová (M)

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84005, Slovakia.

Majlinda Meta (M)

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84005, Slovakia.

Ivan Čavarga (I)

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84005, Slovakia.

Veronika Huntošová (V)

Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenná 5, Košice 04154, Slovakia.

Shubhashis Datta (S)

Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenná 5, Košice 04154, Slovakia.

Pavol Miškovský (P)

Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenná 5, Košice 04154, Slovakia; SAFTRA Photonics s r o., Moldavská cesta 51, Košice 04011, Slovakia.

Juraj Kronek (J)

Department for Biomaterials Research, Polymer Institute of the Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84541, Slovakia.

Boris Bilčík (B)

Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 84005, Slovakia. Electronic address: boris.bilcik@savba.sk.

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Classifications MeSH