Reduced cytotoxicity by mutation of lysine 590 of
Pseudomonas exotoxin
antibody conjugate
immunotoxin
lysine-free protein
protein engineering
Journal
Immunotherapy advances
ISSN: 2732-4303
Titre abrégé: Immunother Adv
Pays: England
ID NLM: 101776979
Informations de publication
Date de publication:
2022
2022
Historique:
received:
08
11
2021
accepted:
17
02
2022
entrez:
3
8
2022
pubmed:
4
8
2022
medline:
4
8
2022
Statut:
epublish
Résumé
Immunotoxins, which are fusion proteins of an antibody fragment and a fragment of a bacterial or a plant toxin, induce apoptosis in target cells by inhibition of protein synthesis. ADP-ribosylating toxins often have few lysine residues in their catalytic domain. As they are the target for ubiquitination, the low number of lysines possibly prevents ubiquitin-dependent degradation of the toxin in the cytosol. To reduce this potential degradation, we aimed to generate a lysine-free (noK),
Identifiants
pubmed: 35919491
doi: 10.1093/immadv/ltac007
pii: ltac007
pmc: PMC9327129
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ltac007Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of the British Society for Immunology.
Références
Int J Mol Sci. 2015 Nov 06;16(11):26706-20
pubmed: 26561808
Sci Transl Med. 2020 Jul 1;12(550):
pubmed: 32611684
Ann Surg Oncol. 2021 Sep;28(9):5252-5262
pubmed: 34019185
Leukemia. 2018 Aug;32(8):1768-1777
pubmed: 30030507
Nat Rev Drug Discov. 2017 May;16(5):315-337
pubmed: 28303026
Int J Mol Sci. 2021 Sep 28;22(19):
pubmed: 34638774
Cancer Immunol Res. 2017 Aug;5(8):685-694
pubmed: 28674083
Blood. 2009 Apr 16;113(16):3792-800
pubmed: 18988862
Cell. 1987 Jan 16;48(1):129-36
pubmed: 3098436
EMBO Rep. 2008 Aug;9(8):802-9
pubmed: 18583986
Methods Mol Biol. 2004;248:503-18
pubmed: 14970517
Biochemistry. 1997 Nov 25;36(47):14577-82
pubmed: 9398176
Biochem J. 1995 Apr 1;307 ( Pt 1):29-37
pubmed: 7717988
Sci Transl Med. 2013 Oct 23;5(208):208ra147
pubmed: 24154601
Blood. 2014 Apr 17;123(16):2470-7
pubmed: 24578503
Int J Mol Sci. 2019 Mar 15;20(6):
pubmed: 30875878
Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5742-7
pubmed: 21436054
Br J Cancer. 2015 Dec 1;113(11):1548-55
pubmed: 26554649
J Immunol. 2011 Feb 1;186(3):1554-63
pubmed: 21178016
Electrophoresis. 1997 Dec;18(15):2714-23
pubmed: 9504803
Oncotarget. 2017 May 9;8(19):30644-30655
pubmed: 28423727
Cancer Res. 2008 Aug 1;68(15):6300-5
pubmed: 18676854
Protein Eng. 2003 Nov;16(11):799-807
pubmed: 14631069
Infect Immun. 2007 Jun;75(6):2946-53
pubmed: 17420233
Annu Rev Biochem. 2001;70:503-33
pubmed: 11395416
Toxins (Basel). 2018 May 21;10(5):
pubmed: 29883379
Leuk Res. 2014 Oct;38(10):1224-9
pubmed: 25127689
Cancers (Basel). 2020 Jun 22;12(6):
pubmed: 32580291
J Neurosurg. 2005 Feb;102(2):267-75
pubmed: 15739554
Bioconjug Chem. 1994 Jan-Feb;5(1):40-6
pubmed: 8199233
Channels (Austin). 2014;8(1):76-83
pubmed: 24088629
J Biol Chem. 1994 May 6;269(18):13398-404
pubmed: 8175770
Dev Cell. 2011 Aug 16;21(2):231-44
pubmed: 21782526
J Cancer Res Clin Oncol. 2015 Dec;141(12):2079-95
pubmed: 25899161
Oncotarget. 2016 Apr 19;7(16):22531-42
pubmed: 26968813
Nat Commun. 2015 Mar 11;6:6536
pubmed: 25758784
J Biol Chem. 1995 Jul 14;270(28):16775-80
pubmed: 7622490
J Mol Biol. 2001 Dec 7;314(4):823-37
pubmed: 11734000
Nat Rev Cancer. 2006 Jul;6(7):559-65
pubmed: 16794638
Sci Rep. 2015 Jun 26;5:10832
pubmed: 26111884
Appl Microbiol Biotechnol. 2017 Apr;101(7):2747-2766
pubmed: 28013405
N Engl J Med. 2019 Apr 25;380(17):1628-1637
pubmed: 31018069
Nat Rev Mol Cell Biol. 2001 Feb;2(2):107-17
pubmed: 11252952
Neurosurg Focus. 2006 Apr 15;20(4):E11
pubmed: 16709016
Immunity. 2000 Jul;13(1):117-27
pubmed: 10933400
Front Microbiol. 2015 Sep 15;6:963
pubmed: 26441897
Science. 2016 Jan 1;351(6268):88-91
pubmed: 26721998
Biochim Biophys Acta. 1993 Dec 21;1154(3-4):237-82
pubmed: 8280743
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):E1135-42
pubmed: 25713356
Clin Cancer Res. 2016 Oct 1;22(19):4913-4922
pubmed: 27114443
J Biol Chem. 2013 Apr 26;288(17):12305-12
pubmed: 23486472