Transmembrane Shuttling of Photosynthetically Produced Electrons to Propel Extracellular Biocatalytic Redox Reactions in a Modular Fashion.

Biocatalysis Cyanobacteria / metabolism Electrons NAD / metabolism NADP / metabolism Oxidation-Reduction Water / metabolism
Biocatalysis Photocatalysis Redox Chemistry Reductions Transmembrane Shuttling

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
04 10 2022
Historique:
received: 03 06 2022
pubmed: 4 8 2022
medline: 28 9 2022
entrez: 3 8 2022
Statut: ppublish

Résumé

Many biocatalytic redox reactions depend on the cofactor NAD(P)H, which may be provided by dedicated recycling systems. Exploiting light and water for NADPH-regeneration as it is performed, e.g. by cyanobacteria, is conceptually very appealing due to its high atom economy. However, the current use of cyanobacteria is limited, e.g. by challenging and time-consuming heterologous enzyme expression in cyanobacteria as well as limitations of substrate or product transport through the cell wall. Here we establish a transmembrane electron shuttling system propelled by the cyanobacterial photosynthesis to drive extracellular NAD(P)H-dependent redox reactions. The modular photo-electron shuttling (MPS) overcomes the need for cloning and problems associated with enzyme- or substrate-toxicity and substrate uptake. The MPS was demonstrated on four classes of enzymes with 19 enzymes and various types of substrates, reaching conversions of up to 99 % and giving products with >99 % optical purity.

Identifiants

DOI: 10.1002/anie.202207971 PMID: 35921249 PMC: PMC9804152
pubmed: 35921249
doi: 10.1002/anie.202207971
pmc: PMC9804152
doi:

Substances chimiques

Water 059QF0KO0R
NAD 0U46U6E8UK
NADP 53-59-8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202207971

Informations de copyright

© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

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Auteurs

Valentina Jurkaš (V)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Florian Weissensteiner (F)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Piera De Santis (P)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.
Department of Engineering, Biological and Chemical Engineering Section, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.

Stephan Vrabl (S)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Frieda A Sorgenfrei (FA)

Austrian Centre of Industrial Biotechnology, c/o, Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Sarah Bierbaumer (S)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Selin Kara (S)

Department of Engineering, Biological and Chemical Engineering Section, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.

Robert Kourist (R)

Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010, Graz, Austria.

Pramod P Wangikar (PP)

Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India, DBT-Pan IIT Centre for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India, Wadhwani Research Centre for Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

Christoph K Winkler (CK)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.

Wolfgang Kroutil (W)

Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.
Field of Excellence BioHealth-, University of Graz, 8010, Graz, Austria.
BioTechMed Graz, 8010, Graz, Austria.
ORCID: 0000-0002-2151-6394

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Biocatalyse › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries à Gram négatif photosynthétiques › Cyanobactéries › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électrons › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NAD › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NADP › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Transmembrane Shuttling of Photosynthetically...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Eau › Transmembrane Shuttling of Photosynthetically...