Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol.
Erwinia tasmaniensis
binding assays
fructosyltransferases
glycosyl hydrolases
levansucrases
microscale thermophoresis
transfructosylation
Journal
Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319
Informations de publication
Date de publication:
01 Aug 2022
01 Aug 2022
Historique:
received:
06
05
2022
accepted:
01
07
2022
entrez:
4
8
2022
pubmed:
5
8
2022
medline:
6
8
2022
Statut:
ppublish
Résumé
Levansucrases are biotechnologically interesting fructosyltransferases due to their potential use in the enzymatic or chemo-enzymatic synthesis of glycosides of non-natural substrates relevant to pharmaceutical applications. The structure of Erwinia tasmaniensis levansucrase in complex with (S)-1,2,4-butanetriol and its biochemical characterization suggests the possible application of short aliphatic moieties containing polyols with defined stereocentres in fructosylation biotechnology. The structural information revealed that (S)-1,2,4-butanetriol mimics the natural substrate. The preference of the protein towards a specific 1,2,4-butanetriol enantiomer was assessed using microscale thermophoresis binding assays. Furthermore, the results obtained and the structural comparison of levansucrases and inulosucrases suggest that the fructose binding modes could differ in fructosyltransferases from Gram-positive and Gram-negative bacteria.
Identifiants
pubmed: 35924596
pii: S2053230X2200680X
doi: 10.1107/S2053230X2200680X
pmc: PMC9350837
doi:
Substances chimiques
1,2,4-butanetriol
0
Anti-Bacterial Agents
0
Butanols
0
Hexosyltransferases
EC 2.4.1.-
levansucrase
EC 2.4.1.10
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-296Subventions
Organisme : Libera Università di Bolzano
ID : 1440
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : LM2018127
Organisme : Instruct-ERIC
ID : PID 12403
Informations de copyright
open access.
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