A SCID Mouse Model To Evaluate the Efficacy of Antivirals against SARS-CoV-2 Infection.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
24 08 2022
Historique:
pubmed: 5 8 2022
medline: 27 8 2022
entrez: 4 8 2022
Statut: ppublish

Résumé

Ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lacks the intrinsic ability to bind to the mouse ACE2 receptor, and therefore establishment of SARS-CoV-2 mouse models has been limited to the use of mouse-adapted viruses or genetically modified mice. Interestingly, some of the variants of concern, such as the Beta B.1.351 variant, show an improved binding to the mouse receptor and hence better replication in different wild-type (WT) mouse species. Here, we describe the establishment of a SARS-CoV-2 Beta B.1.351 variant infection model in male SCID mice as a tool to assess the antiviral efficacy of potential SARS-CoV-2 small-molecule inhibitors. Intranasal infection of male SCID mice with 10

Identifiants

pubmed: 35924921
doi: 10.1128/jvi.00758-22
pmc: PMC9400483
doi:

Substances chimiques

Antiviral Agents 0
RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0075822

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Auteurs

Rana Abdelnabi (R)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Caroline S Foo (CS)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Suzanne J F Kaptein (SJF)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Robbert Boudewijns (R)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Laura Vangeel (L)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Steven De Jonghe (S)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

Dirk Jochmans (D)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
The VirusBank, Leuven, Belgium.
Global Virus Network, Baltimore, Maryland, USA.

Birgit Weynand (B)

KU Leuven Department of Imaging and Pathology, Division of Translational Cell and Tissue Research, Leuven, Belgium.

Johan Neyts (J)

KU Leuven Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Researchgrid.415751.3, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
The VirusBank, Leuven, Belgium.
Global Virus Network, Baltimore, Maryland, USA.

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Classifications MeSH