[Regenerative medicine for the corneal epithelium : Cell therapy from bench to bedside].
Regenerative Medizin für das Hornhautepithel : Zelltherapie in Wissenschaft und Klinik.
Limbal stem cell insufficiency
Limbal stem cell transplantation
Matrices
Reprogramming
Tissue engineering
Journal
Die Ophthalmologie
ISSN: 2731-7218
Titre abrégé: Ophthalmologie
Pays: Germany
ID NLM: 9918402288106676
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
accepted:
31
05
2022
pubmed:
5
8
2022
medline:
8
9
2022
entrez:
4
8
2022
Statut:
ppublish
Résumé
In the case of thermal or caustic burns of the ocular surface, loss of limbal epithelial stem cells leads to compromised self-renewal of the corneal epithelium. This results in permanent loss of vision. In these situations, transplantation of cultured limbal epithelial cells on an amniotic membrane or fibrin gel as substrate (Holoclar®) can help to regenerate the corneal surface. The required cells are obtained from the healthy partner eye, if available. Adult stem cells from other parts of the body potentially serve as alternative cell sources: hair follicles, oral mucosa, mesenchymal stromal cells, or induced pluripotent stem cells (originally, e.g., skin fibroblasts). The reprogramming of such cells can be achieved with the help of transcription factors. In addition, work is being done on biosynthetic or synthetic matrices, which not only serve as substrate material for the transplantation but also support the functional properties of these cells (self-renewal, corneal epithelial-typical phenotype). Bei einer Verbrennung oder Verätzung der Augenoberfläche kann der Verlust von Limbusepithelstammzellen zu einer kompromittierten Selbsterneuerung des Hornhautepithels führen. Hierdurch kommt es zu einem dauerhaften Sehverlust. In diesen Situationen kann die Transplantation von kultivierten Limbusepithelzellen auf einer Amnionmembran oder einem Fibringel als Träger (Holoclar®, Holostem Terapie Avanzate s.r.l., Modena, Italien) zu einer Regeneration der Hornhautoberfläche beitragen. Die erforderlichen Zellen werden dem gesunden Partnerauge entnommen – sofern vorhanden. Alternative Zellquellen sind adulte Stammzellen aus anderen Körperregionen (Haarfollikel, Mundschleimhaut, mesenchymale Stromazellen) oder induzierte pluripotente Stammzellen (ursprünglich z. B. Hautfibroblasten). Das Umprogrammieren solcher Zellen kann unter Zuhilfenahme von Transkriptionsfaktoren erfolgen. Zusätzlich wird an biosynthetischen oder synthetischen Matrices gearbeitet, die nicht nur als Trägermaterial für die Transplantation dienen, sondern auch die Funktionen der Zellen (Selbsterneuerung, hornhautepitheltypischer Phänotyp) unterstützen sollen.
Autres résumés
Type: Publisher
(ger)
Bei einer Verbrennung oder Verätzung der Augenoberfläche kann der Verlust von Limbusepithelstammzellen zu einer kompromittierten Selbsterneuerung des Hornhautepithels führen. Hierdurch kommt es zu einem dauerhaften Sehverlust. In diesen Situationen kann die Transplantation von kultivierten Limbusepithelzellen auf einer Amnionmembran oder einem Fibringel als Träger (Holoclar®, Holostem Terapie Avanzate s.r.l., Modena, Italien) zu einer Regeneration der Hornhautoberfläche beitragen. Die erforderlichen Zellen werden dem gesunden Partnerauge entnommen – sofern vorhanden. Alternative Zellquellen sind adulte Stammzellen aus anderen Körperregionen (Haarfollikel, Mundschleimhaut, mesenchymale Stromazellen) oder induzierte pluripotente Stammzellen (ursprünglich z. B. Hautfibroblasten). Das Umprogrammieren solcher Zellen kann unter Zuhilfenahme von Transkriptionsfaktoren erfolgen. Zusätzlich wird an biosynthetischen oder synthetischen Matrices gearbeitet, die nicht nur als Trägermaterial für die Transplantation dienen, sondern auch die Funktionen der Zellen (Selbsterneuerung, hornhautepitheltypischer Phänotyp) unterstützen sollen.
Identifiants
pubmed: 35925345
doi: 10.1007/s00347-022-01674-8
pii: 10.1007/s00347-022-01674-8
doi:
Types de publication
Journal Article
Review
Langues
ger
Sous-ensembles de citation
IM
Pagination
891-901Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature.
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