A public antibody class recognizes an S2 epitope exposed on open conformations of SARS-CoV-2 spike.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
04 08 2022
04 08 2022
Historique:
received:
30
11
2021
accepted:
22
07
2022
entrez:
4
8
2022
pubmed:
5
8
2022
medline:
9
8
2022
Statut:
epublish
Résumé
Delineating the origins and properties of antibodies elicited by SARS-CoV-2 infection and vaccination is critical for understanding their benefits and potential shortcomings. Therefore, we investigate the SARS-CoV-2 spike (S)-reactive B cell repertoire in unexposed individuals by flow cytometry and single-cell sequencing. We show that ∼82% of SARS-CoV-2 S-reactive B cells harbor a naive phenotype, which represents an unusually high fraction of total human naive B cells (∼0.1%). Approximately 10% of these naive S-reactive B cells share an IGHV1-69/IGKV3-11 B cell receptor pairing, an enrichment of 18-fold compared to the complete naive repertoire. Following SARS-CoV-2 infection, we report an average 37-fold enrichment of IGHV1-69/IGKV3-11 B cell receptor pairing in the S-reactive memory B cells compared to the unselected memory repertoire. This class of B cells targets a previously undefined non-neutralizing epitope on the S2 subunit that becomes exposed on S proteins used in approved vaccines when they transition away from the native pre-fusion state because of instability. These findings can help guide the improvement of SARS-CoV-2 vaccines.
Identifiants
pubmed: 35927266
doi: 10.1038/s41467-022-32232-0
pii: 10.1038/s41467-022-32232-0
pmc: PMC9352689
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
COVID-19 Vaccines
0
Epitopes
0
Immunoglobulin Isotypes
0
Receptors, Antigen, B-Cell
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4539Subventions
Organisme : Bill & Melinda Gates Foundation
ID : INV-002022
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-004923
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-024617
Pays : United States
Informations de copyright
© 2022. The Author(s).
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