Nasopharyngeal microbiota profiling of pregnant women with SARS-CoV-2 infection.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 08 2022
04 08 2022
Historique:
received:
05
01
2022
accepted:
27
07
2022
entrez:
4
8
2022
pubmed:
5
8
2022
medline:
9
8
2022
Statut:
epublish
Résumé
We aimed to analyze the nasopharyngeal microbiota profiles in pregnant women with and without SARS-CoV-2 infection, considered a vulnerable population during COVID-19 pandemic. Pregnant women were enrolled from a multicenter prospective population-based cohort during the first SARS-CoV-2 wave in Spain (March-June 2020 in Barcelona, Spain) in which the status of SARS-CoV-2 infection was determined by nasopharyngeal RT-PCR and antibodies in peripheral blood. Women were randomly selected for this cross-sectional study on microbiota. DNA was extracted from nasopharyngeal swab samples, and the V3-V4 region of the 16S rRNA of bacteria was amplified using region-specific primers. The differential abundance of taxa was tested, and alpha/beta diversity was evaluated. Among 76 women, 38 were classified as positive and 38 as negative for SARS-CoV-2 infection. All positive women were diagnosed by SARS-CoV-2 IgG and IgM/IgA antibodies, and 14 (37%) also had a positive RT-PCR. The overall composition of the nasopharyngeal microbiota differ in pregnant women with SARS-CoV-2 infection (positive SARS-CoV-2 antibodies), compared to those without the infection (negative SARS-CoV-2 antibodies) (p = 0.001), with a higher relative abundance of the Tenericutes and Bacteroidetes phyla and a higher abundance of the Prevotellaceae family. Infected women presented a different pattern of microbiota profiling due to beta diversity and higher richness (observed ASV < 0.001) and evenness (Shannon index < 0.001) at alpha diversity. These changes were also present in women after acute infection, as revealed by negative RT-PCR but positive SARS-CoV-2 antibodies, suggesting a potential association between SARS-CoV-2 infection and long-lasting shift in the nasopharyngeal microbiota. No significant differences were reported in mild vs. severe cases. This is the first study on nasopharyngeal microbiota during pregnancy. Pregnant women with SARS-CoV-2 infection had a different nasopharyngeal microbiota profile compared to negative cases.
Identifiants
pubmed: 35927569
doi: 10.1038/s41598-022-17542-z
pii: 10.1038/s41598-022-17542-z
pmc: PMC9352760
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin M
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13404Informations de copyright
© 2022. The Author(s).
Références
Hum Microb J. 2020 Aug;17:100073
pubmed: 32835135
J Infect. 2020 Sep;81(3):e64-e67
pubmed: 32579991
Sci Rep. 2021 Sep 23;11(1):18984
pubmed: 34556736
PLoS One. 2021 Jun 23;16(6):e0253293
pubmed: 34161373
Front Microbiol. 2021 Mar 17;12:637430
pubmed: 33815323
Front Microbiol. 2020 Jul 31;11:1840
pubmed: 32849438
Lancet. 2020 Aug 22;396(10250):530-531
pubmed: 32771084
BMC Biol. 2019 Nov 7;17(1):87
pubmed: 31699101
Microb Ecol Health Dis. 2015 May 29;26:27663
pubmed: 26028277
Sci Rep. 2021 Apr 26;11(1):8922
pubmed: 33903709
PLoS One. 2010 Mar 10;5(3):e9490
pubmed: 20224823
JAMA Pediatr. 2021 Aug 1;175(8):817-826
pubmed: 33885740
Nat Biotechnol. 2019 Aug;37(8):852-857
pubmed: 31341288
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Int J Environ Res Public Health. 2021 Feb 23;18(4):
pubmed: 33672177
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Bioinformatics. 2017 Mar 1;33(5):782-783
pubmed: 28025202
Sci Rep. 2018 Jan 22;8(1):1296
pubmed: 29358754
Clin Infect Dis. 2020 Jul 28;71(15):713-720
pubmed: 32129843
Cell Host Microbe. 2018 Jul 11;24(1):146-154.e4
pubmed: 30001517
Am J Reprod Immunol. 2010 Jun;63(6):425-33
pubmed: 20367629
PLoS One. 2019 Jan 9;14(1):e0207898
pubmed: 30625134
Biol Proced Online. 2021 Jun 1;23(1):10
pubmed: 34058978
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Nat Rev Microbiol. 2017 May;15(5):259-270
pubmed: 28316330
Biomed Res Int. 2018 Nov 19;2018:6362716
pubmed: 30581863
Clin Infect Dis. 2020 Aug 22;71(5):1195-1203
pubmed: 31562814
Ann Epidemiol. 2019 Sep;37:51-56.e6
pubmed: 31451313
Nat Microbiol. 2021 Oct;6(10):1245-1258
pubmed: 34465900
Clin Infect Dis. 2021 Nov 16;73(10):1768-1775
pubmed: 33556958
Postgrad Med J. 2021 May;97(1147):312-320
pubmed: 32978337
Cell Host Microbe. 2018 Jul 11;24(1):133-145.e5
pubmed: 30001516
Pediatr Infect Dis J. 2021 Jan;40(1):16-21
pubmed: 33055502
ISME J. 2011 Feb;5(2):169-72
pubmed: 20827291
Microbiome. 2016 Jul 01;4(1):34
pubmed: 27364497
N Engl J Med. 2020 Apr 16;382(16):1564-1567
pubmed: 32182409
PLoS One. 2020 Jul 13;15(7):e0235498
pubmed: 32658916
N Engl J Med. 2020 May 28;382(22):2163-2164
pubmed: 32283004
Cell. 2012 Aug 3;150(3):470-80
pubmed: 22863002
Sci Rep. 2019 Apr 16;9(1):6175
pubmed: 30992494
Front Cell Infect Microbiol. 2021 Feb 15;11:625581
pubmed: 33659220
Biol Proced Online. 2020 Jul 25;22:18
pubmed: 32728349
Appl Environ Microbiol. 2003 Jan;69(1):18-23
pubmed: 12513972
Nat Commun. 2015 Jun 23;6:7320
pubmed: 26102221