Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured

Animals Argonaute Proteins / genetics Drosophila / genetics Drosophila Proteins / genetics RNA RNA Interference RNA-Induced Silencing Complex / genetics
Cell-based Assays Gene Expression Molecular Biology Molecular/Chemical Probes Protein Biochemistry

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 5 8 2022
pubmed: 6 8 2022
medline: 9 8 2022
Statut: epublish

Résumé

Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol

Identifiants

DOI: 10.1016/j.xpro.2022.101596 PMID: 35928005 PMC: PMC9344023
pubmed: 35928005
doi: 10.1016/j.xpro.2022.101596
pii: S2666-1667(22)00476-2
pmc: PMC9344023
pii:
doi:

Substances chimiques

AGO1 protein, Drosophila 0
Argonaute Proteins 0
Drosophila Proteins 0
RNA-Induced Silencing Complex 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101596

Informations de copyright

© 2022 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

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pubmed: 34942118

Auteurs

Yajie Gao (Y)

Institute of Biomedical Research, Yunnan University, Kunming 650500, China. Electronic address: gaoyajie@ynu.edu.cn.

Yuanxiang Zhu (Y)

Institute of Biomedical Research, Yunnan University, Kunming 650500, China.

Qinmiao Sun (Q)

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute of Stem Cells and Regeneration, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: qinmiaosun@ioz.ac.cn.

Dahua Chen (D)

Institute of Biomedical Research, Yunnan University, Kunming 650500, China; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: chendh@ynu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Complexe réprimant l'expression de l'ARN › Protéines Argonaute › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Optimized protocols for RNA-induced silencing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Complexe réprimant l'expression de l'ARN › Optimized protocols for RNA-induced silencing...