Mating success of timed pregnancies in Sprague Dawley rats: Considerations for execution.


Journal

Reproductive biology
ISSN: 2300-732X
Titre abrégé: Reprod Biol
Pays: Poland
ID NLM: 101160559

Informations de publication

Date de publication:
Sep 2022
Historique:
received: 23 04 2022
revised: 28 07 2022
accepted: 29 07 2022
pubmed: 6 8 2022
medline: 31 8 2022
entrez: 5 8 2022
Statut: ppublish

Résumé

This study compares three different mating techniques in Sprague-Dawley rats, using the pregnancy rate as the main indicator of success. It provides recommendations for timed-pregnancy experiments to achieve an appropriate sample size for the study of human pregnancy disorders. The implementation of a preconditioning phase, determination of the estrous cycle, the use of two mating strategies (Lee-Boot and Whitten effect), female: male mating ratios, and cohabitation duration should be considered as they improve the mating success rate.

Identifiants

pubmed: 35930886
pii: S1642-431X(22)00081-X
doi: 10.1016/j.repbio.2022.100682
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

100682

Informations de copyright

Copyright © 2022 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.

Auteurs

Rebecca Reddy (R)

Dept of Basic Medical Science, Faculty of Health Sciences, Durban University of Technology, Durban, South Africa.

Virushka Pillay (V)

Dept of Basic Medical Science, Faculty of Health Sciences, Durban University of Technology, Durban, South Africa.

Sooraj Baijnath (S)

School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Sanil D Singh (SD)

Biomedical Resource Unit, University of KwaZulu-Natal, Westville Campus, Durban, South Africa.

Sapna Ramdin (S)

Dept of Basic Medical Science, Faculty of Health Sciences, Durban University of Technology, Durban, South Africa.

Thajasvarie Naicker (T)

Optics and Imaging Centre, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, South Africa.

Nalini Govender (N)

Dept of Basic Medical Science, Faculty of Health Sciences, Durban University of Technology, Durban, South Africa. Electronic address: nalinip@dut.ac.za.

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Classifications MeSH