Thrombosis-related circulating miR-16-5p is associated with disease severity in patients hospitalised for COVID-19.


Journal

RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328

Informations de publication

Date de publication:
01 2022
Historique:
entrez: 8 8 2022
pubmed: 9 8 2022
medline: 10 8 2022
Statut: ppublish

Résumé

SARS-CoV-2 tropism for the ACE2 receptor, along with the multifaceted inflammatory reaction, is likely to drive the generalized hypercoagulable and thrombotic state seen in patients with COVID-19. Using the original bioinformatic workflow and network medicine approaches we reanalysed four coronavirus-related expression datasets and performed co-expression analysis focused on thrombosis and ACE2 related genes. We identified microRNAs (miRNAs) which play role in ACE2-related thrombosis in coronavirus infection and further, we validated the expressions of precisely selected miRNAs-related to thrombosis (miR-16-5p, miR-27a-3p, let-7b-5p and miR-155-5p) in 79 hospitalized COVID-19 patients and 32 healthy volunteers by qRT-PCR. Consequently, we aimed to unravel whether bioinformatic prioritization could guide selection of miRNAs with a potential of diagnostic and prognostic biomarkers associated with disease severity in patients hospitalized for COVID-19. In bioinformatic analysis, we identified EGFR, HSP90AA1, APP, TP53, PTEN, UBC, FN1, ELAVL1 and CALM1 as regulatory genes which could play a pivotal role in COVID-19 related thrombosis. We also found miR-16-5p, miR-27a-3p, let-7b-5p and miR-155-5p as regulators in the coagulation and thrombosis process.

Identifiants

pubmed: 35938548
doi: 10.1080/15476286.2022.2100629
pmc: PMC9361765
doi:

Substances chimiques

Amyloid beta-Peptides 0
Biomarkers 0
Heat-Shock Proteins 0
MIRN16 microRNA, human 0
MicroRNAs 0
Angiotensin-Converting Enzyme 2 EC 3.4.17.23

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

963-979

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Auteurs

Ceren Eyileten (C)

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Center for Preclinical Research and Technology CEPT, Warsaw, Poland.
Genomics Core Facility, Centre of New Technologies, University of Warsaw, Warsaw, Poland.

Zofia Wicik (Z)

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Center for Preclinical Research and Technology CEPT, Warsaw, Poland.
Center for Mathematics, Computing and Cognition, Federal University of ABC, Santo André Brazil.

Sérgio N Simões (SN)

Department of Informatics, Federal Institute of Espírito Santo, Serra, Brazil.

David C Martins-Jr (DC)

Center for Mathematics, Computing and Cognition, Federal University of ABC, Santo André Brazil.

Krzysztof Klos (K)

Department of Infectious Diseases and Allergology - Military Institute of Medicine, Warsaw, Poland.

Wojciech Wlodarczyk (W)

Department of Infectious Diseases and Allergology - Military Institute of Medicine, Warsaw, Poland.

Alice Assinger (A)

Department of Vascular Biology and Thrombosis Research, Center of Physiology and Pharmacology, Medical University of Vienna, Austria.

Dariusz Soldacki (D)

Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland.

Andrzej Chcialowski (A)

Department of Infectious Diseases and Allergology - Military Institute of Medicine, Warsaw, Poland.

Jolanta M Siller-Matula (JM)

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Center for Preclinical Research and Technology CEPT, Warsaw, Poland.
Department of Internal Medicine II, Division of Cardiology, Medical University of Vienna, Vienna, Austria.

Marek Postula (M)

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Center for Preclinical Research and Technology CEPT, Warsaw, Poland.

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