Evaluation of Prototype CE-MS Interfaces.
CE-MS interfacing
Design of experiments
Electrospray optimization
Low-flow sheath-liquid interface
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
8
8
2022
pubmed:
9
8
2022
medline:
11
8
2022
Statut:
ppublish
Résumé
Capillary electrophoresis-mass spectrometry (CE-MS) coupling is a powerful analytical solution bringing together the separation power of CE and the wealth of chemical information afforded by MS. Nevertheless, interfaces making the hyphenation of both techniques possible have always been the subject of a quest for improvement by their users in search for more sensitive and robust setups. This fact has led to numerous technical developments and new interface designs claiming to outrival existing approaches in different aspects. Nevertheless, the task of evaluating and comparing a new interface to previous solutions is not always straightforward. Issued from our own experience in the field, we herein propose a protocol to optimize the operation parameters of a new CE-MS interface design, assess its analytical performance, and compare it to a reference interface if desired. Electrospray stability, sensitivity, reproducibility, and robustness are practically evaluated as key elements of the process.
Identifiants
pubmed: 35941475
doi: 10.1007/978-1-0716-2493-7_1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-13Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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