Electrokinetic characterization of hybrid NOA 81-glass microchips: Application to protein microchip electrophoresis with indirect fluorescence detection.


Journal

Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476

Informations de publication

Date de publication:
10 2022
Historique:
revised: 08 07 2022
received: 06 03 2022
accepted: 15 07 2022
pubmed: 11 8 2022
medline: 19 10 2022
entrez: 10 8 2022
Statut: ppublish

Résumé

A low-cost and straightforward hybrid NOA (Norland optical adhesive) 81-glass microchip electrophoresis device was designed and developed for protein separation using indirect fluorescence detection. This new microchip was first characterized in terms of surface charge density via electroosmotic mobility measurement and stability over time. A systematic determination of the electroosmotic mobility (μ

Identifiants

pubmed: 35948488
doi: 10.1002/elps.202200057
doi:

Substances chimiques

Proteins 0
Sulfhydryl Compounds 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2044-2048

Informations de copyright

© 2022 Wiley-VCH GmbH.

Références

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Auteurs

Menel Ben Frej (M)

Institute of Chemistry for Life and Health Sciences i-CLeHS, Chimie ParisTech-PSL/CNRS 8060 , Paris, France.

Fanny d'Orlyé (F)

Institute of Chemistry for Life and Health Sciences i-CLeHS, Chimie ParisTech-PSL/CNRS 8060 , Paris, France.

Gerson F Duarte-Junior (GF)

Institute of Chemistry for Life and Health Sciences i-CLeHS, Chimie ParisTech-PSL/CNRS 8060 , Paris, France.
Instituto de Química, Universidade Federal de Goiás, Goiânia, Brazil.

Wendell K T Coltro (WKT)

Instituto de Química, Universidade Federal de Goiás, Goiânia, Brazil.

Anne Varenne (A)

Institute of Chemistry for Life and Health Sciences i-CLeHS, Chimie ParisTech-PSL/CNRS 8060 , Paris, France.

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