Coenzyme Q10, Biochanin A and Phloretin Attenuate Cr(VI)-Induced Oxidative Stress and DNA Damage by Stimulating Nrf2/HO-1 Pathway in the Experimental Model.
Biochanin A
Coenzyme Q10
Cr(VI)
Natural compounds
Oxidative stress
Phloretin
Journal
Biological trace element research
ISSN: 1559-0720
Titre abrégé: Biol Trace Elem Res
Pays: United States
ID NLM: 7911509
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
12
05
2022
accepted:
12
07
2022
pubmed:
12
8
2022
medline:
22
3
2023
entrez:
11
8
2022
Statut:
ppublish
Résumé
Hexavalent chromium [Cr(VI)] has emerged as a prevailing environmental and occupational contaminant over the past few decades. However, the knowledge is sparse regarding Cr(VI)-induced neurological aberrations, and its remediation through natural bioactive compounds has not been fully explored. This study intended to probe the possible invigorative effects of nutraceuticals such as coenzyme Q10 (CoQ10), biochanin A (BCA), and phloretin (PHL) on Cr(VI) intoxicated Swiss albino mice with special emphasis on Nrf2/HO-1/NQO1 gene expressions. Mice received potassium dichromate (75 ppm) through drinking water and were simultaneously co-treated intraperitoneally with CoQ10 (10 mg/kg), BCA, and PHL (50 mg/kg) each for 30-day treatment period. The statistics highlight the elevated levels of lipid peroxidation (LPO) and protein carbonyl content (PCC) with a concomitant reduction in the superoxide dismutase (SOD), glutathione-S-transferase (GST), reduced glutathione (GSH), total thiols (TT), catalase (CAT), and cholinesterase activities in the Cr(VI)-exposed mice. The collateral assessment of DNA fragmentation, DNA breakages, and induced histological alterations was in conformity with the above findings in conjugation with the dysregulation in the Nrf2 and associated downstream HO-1 and NQO1 gene expressions. Co-treatment with the selected natural compounds reversed the above-altered parameters significantly, thereby bringing cellular homeostasis in alleviating the Cr(VI)-induced conciliated impairments. Our study demonstrated that the combination of different bioactive compounds shields the brain better against Cr(VI)-induced neurotoxicity by revoking the oxidative stress-associated manifestations. These compounds may represent a new potential combination therapy due to their ability to modulate the cellular antioxidant responses by upregulating the Nrf2/HO-1/NQO1 signaling pathway against Cr(VI)-exposed population. HIGHLIGHTS: Cr(VI)-associated heavy metal exposure poses a significant threat to the environment, especially to living organisms. Cr(VI) exposure for 30 days resulted in the free radical's generation that caused neurotoxicity in the Swiss albino mice. Natural compounds such as coenzyme Q10, biochanin A, and phloretin counteracted the neurotoxic effect due to Cr(VI) exposure in scavenging of free radicals by enhancing Nrf2/HO-1/NQO1 gene expressions in maintaining the cellular homeostasis.
Identifiants
pubmed: 35953644
doi: 10.1007/s12011-022-03358-5
pii: 10.1007/s12011-022-03358-5
doi:
Substances chimiques
chromium hexavalent ion
18540-29-9
coenzyme Q10
EJ27X76M46
biochanin A
U13J6U390T
NF-E2-Related Factor 2
0
Phloretin
S5J5OE47MK
Antioxidants
0
Chromium
0R0008Q3JB
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2427-2441Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Aebi H (1974) Catalase. Academic Press, Methods Of Enzymatic Analysis. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
Aldhahri RS, Alghamdi BS, Alzahrani NA, Bahaidrah KA, Alsufiani HM, Mansouri RA and Ashraf GM (2022) Biochanin A Improves Memory Decline And Brain Pathology In Cuprizone-Induced Mouse Model Of Multiple Sclerosis. Behav Sci (Basel) 12
Atici S, Cinel L, Cinel I, Doruk N, Aktekin M, Akca A, Camdeviren H, Oral U (2004) Opioid Neurotoxicity: Comparison Of Morphine And Tramadol In An Experimental Rat Model. Int J Neurosci 114:1001–1011
pubmed: 15527204
doi: 10.1080/00207450490461314
Atsdr U (2012) Toxicological Profile For Chromium. Us Department Of Health And Human Services, Public Health Service. https://wwwn.Cdc.Gov/Tsp/Toxprofiles/Toxprofiles.Aspx
Bagchi D, Balmoori J, Bagchi M, Ye X, Williams CB, Stohs SJ (2002) Comparative Effects Of Tcdd, Endrin, Naphthalene And Chromium (Vi) On Oxidative Stress And Tissue Damage In The Liver And Brain Tissues Of Mice. Toxicology 175:73–82
pubmed: 12049837
doi: 10.1016/S0300-483X(02)00062-8
Bagchi D, Stohs SJ, Downs BW, Bagchi M, Preuss HG (2002) Cytotoxicity And Oxidative Mechanisms Of Different Forms Of Chromium. Toxicology 180:5–22
pubmed: 12324196
doi: 10.1016/S0300-483X(02)00378-5
Bagchi D, Vuchetich PJ, Bagchi M, Hassoun EA, Tran MX, Tang L, Stohs SJ (1997) Induction Of Oxidative Stress By Chronic Administration Of Sodium Dichromate [Chromium Vi] And Cadmium Chloride [Cadmium Ii] To Rats. Free Radic Biol Med 22:471–478
pubmed: 8981039
doi: 10.1016/S0891-5849(96)00352-8
Bailey MM, Boohaker JG, Jernigan PL, Townsend MB, Sturdivant J, Rasco JF, Vincent JB, Hood RD (2008) Effects Of Pre- And Postnatal Exposure To Chromium Picolinate Or Picolinic Acid On Neurological Development In Cd-1 Mice. Biol Trace Elem Res 124:70–82
pubmed: 18408898
doi: 10.1007/s12011-008-8124-9
Barceloux DG (1999) Chromium. J Toxicol Clin Toxicol 37:173–194
pubmed: 10382554
doi: 10.1081/CLT-100102418
Bosgelmez Ii, Soylemezoglu T, Guvendik G (2008) The Protective And Antidotal Effects Of Taurine On Hexavalent Chromium-Induced Oxidative Stress In Mice Liver Tissue. Biol Trace Elem Res 125:46–58
pubmed: 18528645
doi: 10.1007/s12011-008-8154-3
Buege JA, Aust SD (1978) Microsomal Lipid Peroxidation. Methods Enzymol 52:302–310
pubmed: 672633
doi: 10.1016/S0076-6879(78)52032-6
Chen H, Mu L, Cao J, Mu J, Klerks PL, Luo Y, Guo Z, Xie L (2016) Accumulation And Effects Of Cr(Vi) In Japanese Medaka (Oryzias Latipes) During Chronic Dissolved And Dietary Exposures. Aquat Toxicol 176:208–216
pubmed: 27162070
doi: 10.1016/j.aquatox.2016.05.002
Dashti A, Soodi M, Amani N (2016) Cr (Vi) Induced Oxidative Stress And Toxicity In Cultured Cerebellar Granule Neurons At Different Stages Of Development And Protective Effect Of Rosmarinic Acid. Environ Toxicol 31:269–277
pubmed: 25213303
doi: 10.1002/tox.22041
Ellman GL (1959) Tissue Sulfhydryl Groups. Arch Biochem Biophys 82:70–77
pubmed: 13650640
doi: 10.1016/0003-9861(59)90090-6
Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM (1961) A New And Rapid Colorimetric Determination Of Acetylcholinesterase Activity. Biochem Pharmacol 7:88–95
pubmed: 13726518
doi: 10.1016/0006-2952(61)90145-9
Guilhermino L, Soares AM, Carvalho AP, Lopes MC (1998) Correlation Between Whole Blood Cholinesterase Activity And Cerebral Cortex Cholinesterase Activity In Rats Treated With Parathion. Chemosphere 37:1385–1393
pubmed: 9734325
doi: 10.1016/S0045-6535(98)00129-5
Guo M, Lu H, Qin J, Qu S, Wang W, Guo Y, Liao W, Song M, Chen J, Wang Y (2019) Biochanin A Provides Neuroprotection Against Cerebral Ischemia/Reperfusion Injury By Nrf2-Mediated Inhibition Of Oxidative Stress And Inflammation Signaling Pathway In Rats. Med Sci Monit 25:8975–8983
pubmed: 31767824
pmcid: 6896748
doi: 10.12659/MSM.918665
Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-Transferases. The First Enzymatic Step In Mercapturic Acid Formation. J Biol Chem 249:7130–7139
pubmed: 4436300
doi: 10.1016/S0021-9258(19)42083-8
Han B, Li S, Lv Y, Yang D, Li J, Yang Q, Wu P, Lv Z, Zhang Z (2019) Dietary Melatonin Attenuates Chromium-Induced Lung Injury Via Activating The Sirt1/Pgc-1alpha/Nrf2 Pathway. Food Funct 10:5555–5565
pubmed: 31429458
doi: 10.1039/C9FO01152H
Hao P, Zhu Y, Wang S, Wan H, Chen P, Wang Y, Cheng Z, Liu Y, Liu J (2017) Selenium Administration Alleviates Toxicity Of Chromium(Vi) In The Chicken Brain. Biol Trace Elem Res 178:127–135
pubmed: 28013456
doi: 10.1007/s12011-016-0915-9
Hu ML (1994) Measurement Of Protein Thiol Groups And Glutathione In Plasma. Methods Enzymol 233:380–385
pubmed: 8015473
doi: 10.1016/S0076-6879(94)33044-1
Itoh K, Wakabayashi N, Katoh Y, Ishii T, Igarashi K, Engel JD, Yamamoto M (1999) Keap1 Represses Nuclear Activation Of Antioxidant Responsive Elements By Nrf2 Through Binding To The Amino-Terminal Neh2 Domain. Genes Dev 13:76–86
pubmed: 9887101
pmcid: 316370
doi: 10.1101/gad.13.1.76
Jin Y, Zhang S, Tao R, Huang J, He X, Qu L, Fu Z (2016) Oral Exposure Of Mice To Cadmium (Ii), Chromium (Vi) And Their Mixture Induce Oxidative- And Endoplasmic Reticulum-Stress Mediated Apoptosis In The Livers. Environ Toxicol 31:693–705
pubmed: 25409916
doi: 10.1002/tox.22082
Kadiiska MB, Xiang QH, Mason RP (1994) In Vivo Free Radical Generation By Chromium(Vi): An Electron Spin Resonance Spin-Trapping Investigation. Chem Res Toxicol 7:800–805
pubmed: 7696535
doi: 10.1021/tx00042a013
Levine RL, Berlett BS, Moskovitz J, Mosoni L, Stadtman ER (1999) Methionine Residues May Protect Proteins From Critical Oxidative Damage. Mech Ageing Dev 107:323–332
pubmed: 10360685
doi: 10.1016/S0047-6374(98)00152-3
Li J, Yu Z, Han B, Li S, Lv Y, Wang X, Yang Q, Wu P, Liao Y, Qu B, Zhang Z (2022) Activation Of The Gpx4/Tlr4 Signaling Pathway Participates In The Alleviation Of Selenium Yeast On Deltamethrin-Provoked Cerebrum Injury In Quails. Mol Neurobiol 59:2946–2961
pubmed: 35247140
doi: 10.1007/s12035-022-02744-3
Li S, Wu P, Han B, Yang Q, Wang X, Li J, Deng N, Han B, Liao Y, Liu Y, Zhang Z (2022) Deltamethrin Induces Apoptosis In Cerebrum Neurons Of Quail Via Promoting Endoplasmic Reticulum Stress And Mitochondrial Dysfunction. Environ Toxicol 37:2033–2043
pubmed: 35446475
doi: 10.1002/tox.23548
Li X, He S, Zhou J, Yu X, Li L, Liu Y, Li W (2021) Cr (Vi) Induces Abnormalities In Glucose And Lipid Metabolism Through Ros/Nrf2 Signaling. Ecotoxicol Environ Saf 219:112320
pubmed: 33991932
doi: 10.1016/j.ecoenv.2021.112320
Liu Y, Zhang L, Liang J (2015) Activation Of The Nrf2 Defense Pathway Contributes To Neuroprotective Effects Of Phloretin On Oxidative Stress Injury After Cerebral Ischemia/Reperfusion In Rats. J Neurol Sci 351:88–92
pubmed: 25770876
doi: 10.1016/j.jns.2015.02.045
Lu J, Jiang H, Liu B, Baiyun R, Li S, Lv Y, Li D, Qiao S, Tan X, Zhang Z (2018) Grape Seed Procyanidin Extract Protects Against Pb-Induced Lung Toxicity By Activating The Ampk/Nrf2/P62 Signaling Axis. Food Chem Toxicol 116:59–69
pubmed: 29630945
doi: 10.1016/j.fct.2018.03.034
Mahmoud AM, Abd El-Twab SM (2017) Caffeic Acid Phenethyl Ester Protects The Brain Against Hexavalent Chromium Toxicity By Enhancing Endogenous Antioxidants And Modulating The Jak/Stat Signaling Pathway. Biomed Pharmacother 91:303–311
pubmed: 28463793
doi: 10.1016/j.biopha.2017.04.073
Majumdar A, Nirwane A, Kamble R (2014) New Evidences Of Neurotoxicity Of Aroclor 1254 In Mice Brain: Potential Of Coenzyme Q10 In Abating The Detrimental Outcomes. Environ Health Toxicol 29:E2014001
pubmed: 24683537
pmcid: 3965848
doi: 10.5620/eht.2014.29.e2014001
Marklund S, Marklund G (1974) Involvement Of The Superoxide Anion Radical In The Autoxidation Of Pyrogallol And A Convenient Assay For Superoxide Dismutase. Eur J Biochem 47:469–474
pubmed: 4215654
doi: 10.1111/j.1432-1033.1974.tb03714.x
Mary Momo CM, Ferdinand N, Omer Bebe NK, Alexane Marquise MN, Augustave K, Bertin Narcisse V, Herve T and Joseph T (2019) Oxidative Effects Of Potassium Dichromate On Biochemical, Hematological Characteristics, And Hormonal Levels In Rabbit Doe (Oryctolagus Cuniculus). Vet Sci 6
Michiels C, Raes M, Toussaint O, Remacle J (1994) Importance Of Se-Glutathione Peroxidase, Catalase, And Cu/Zn-Sod For Cell Survival Against Oxidative Stress. Free Radic Biol Med 17:235–248
pubmed: 7982629
doi: 10.1016/0891-5849(94)90079-5
Nyariki JN, Ochola LA, Jillani NE, Nyamweya NO, Amwayi PE, Yole DS, Azonvide L, Isaac AO (2019) Oral Administration Of Coenzyme Q10 Protects Mice Against Oxidative Stress And Neuro-Inflammation During Experimental Cerebral Malaria. Parasitol Int 71:106–120
pubmed: 30981893
doi: 10.1016/j.parint.2019.04.010
O’flaherty EJ, Kerger BD, Hays SM, Paustenbach DJ (2001) A Physiologically Based Model For The Ingestion Of Chromium(Iii) And Chromium(Vi) By Humans. Toxicol Sci 60:196–213
pubmed: 11248132
doi: 10.1093/toxsci/60.2.196
Patlolla AK, Barnes C, Yedjou C, Velma VR, Tchounwou PB (2009) Oxidative Stress, Dna Damage, And Antioxidant Enzyme Activity Induced By Hexavalent Chromium In Sprague-Dawley Rats. Environ Toxicol 24:66–73
pubmed: 18508361
pmcid: 2769560
doi: 10.1002/tox.20395
Pratush A, Kumar A, Hu Z (2018) Adverse Effect Of Heavy Metals (As, Pb, Hg, And Cr) On Health And Their Bioremediation Strategies: A Review. Int Microbiol 21:97–106
pubmed: 30810952
doi: 10.1007/s10123-018-0012-3
Prince PD, Rodriguez Lanzi C, Fraga CG, Galleano M (2019) Dietary (-)-Epicatechin Affects Nf-Kappab Activation And Nadph Oxidases In The Kidney Cortex Of High-Fructose-Fed Rats. Food Funct 10:26–32
pubmed: 30604799
doi: 10.1039/C8FO02230E
Ray RR (2016) Adverse Hematological Effects Of Hexavalent Chromium: An Overview. Interdiscip Toxicol 9:55–65
pubmed: 28652847
doi: 10.1515/intox-2016-0007
Salama A, Hegazy R, Hassan A (2016) Intranasal Chromium Induces Acute Brain And Lung Injuries In Rats: Assessment Of Different Potential Hazardous Effects Of Environmental And Occupational Exposure To Chromium And Introduction Of A Novel Pharmacological And Toxicological Animal Model. PLoS ONE 11:E0168688
pubmed: 27997619
pmcid: 5173240
doi: 10.1371/journal.pone.0168688
Sanchez-Diaz G, Escobar F, Badland H, Arias-Merino G, Posada De La Paz M, and Alonso-Ferreira V (2018) Geographic Analysis Of Motor Neuron Disease Mortality And Heavy Metals Released To Rivers In Spain. Int J Environ Res Public Health 15
Shipkowski KA, Sheth CM, Smith MJ, Hooth MJ, White KL Jr, Germolec DR (2017) Assessment Of Immunotoxicity In Female Fischer 344/N And Sprague Dawley Rats And Female B6c3f1 Mice Exposed To Hexavalent Chromium Via The Drinking Water. J Immunotoxicol 14:215–227
pubmed: 29141474
doi: 10.1080/1547691X.2017.1394932
Singh G, Thaker R, Sharma A, Parmar D (2021) Therapeutic Effects Of Biochanin A, Phloretin, And Epigallocatechin-3-Gallate In Reducing Oxidative Stress In Arsenic-Intoxicated Mice. Environ Sci Pollut Res Int 28:20517–20536
pubmed: 33410021
doi: 10.1007/s11356-020-11740-w
Singh NP, Mccoy MT, Tice RR, Schneider EL (1988) A Simple Technique For Quantitation Of Low Levels Of Dna Damage In Individual Cells. Exp Cell Res 175:184–191
pubmed: 3345800
doi: 10.1016/0014-4827(88)90265-0
Singh P, Chowdhuri DK (2017) Environmental Presence Of Hexavalent But Not Trivalent Chromium Causes Neurotoxicity In Exposed Drosophila Melanogaster. Mol Neurobiol 54:3368–3387
pubmed: 27167131
doi: 10.1007/s12035-016-9909-z
Sirvio J, Soininen HS, Kutvonen R, Hyttinen JM, Helkala EL, Riekkinen PJ (1987) Acetyl- And Butyrylcholinesterase Activity In The Cerebrospinal Fluid Of Patients With Parkinson’s Disease. J Neurol Sci 81:273–279
pubmed: 3694232
doi: 10.1016/0022-510X(87)90102-X
Soudani N, Troudi A, Amara IB, Bouaziz H, Boudawara T, Zeghal N (2012) Ameliorating Effect Of Selenium On Chromium (Vi)-Induced Oxidative Damage In The Brain Of Adult Rats. J Physiol Biochem 68:397–409
pubmed: 22351115
doi: 10.1007/s13105-012-0152-4
Suzuki YJ, Carini M, Butterfield DA (2010) Protein Carbonylation. Antioxid Redox Signal 12:323–325
pubmed: 19743917
pmcid: 2821144
doi: 10.1089/ars.2009.2887
Thompson CM, Proctor DM, Haws LC, Hebert CD, Grimes SD, Shertzer HG, Kopec AK, Hixon JG, Zacharewski TR, Harris MA (2011) Investigation Of The Mode Of Action Underlying The Tumorigenic Response Induced In B6c3f1 Mice Exposed Orally To Hexavalent Chromium. Toxicol Sci 123:58–70
pubmed: 21712504
pmcid: 3164443
doi: 10.1093/toxsci/kfr164
Travacio M, Polo JM, Llesuy S (2001) Chromium (Vi) Induces Oxidative Stress In The Mouse Brain. Toxicology 162:139–148
pubmed: 11337112
doi: 10.1016/S0300-483X(00)00423-6
Tripathi S, Fhatima S, Parmar D, Singh DP, Mishra S, Mishra R and Singh G (2022) Therapeutic Effects Of Coenzymeq10, Biochanin A And Phloretin Against Arsenic And Chromium Induced Oxidative Stress In Mouse (Mus Musculus) Brain. 3 Biotech 12:1–13
Wang J, He C, Wu WY, Chen F, Wu YY, Li WZ, Chen HQ, Yin YY (2015) Biochanin A Protects Dopaminergic Neurons Against Lipopolysaccharide-Induced Damage And Oxidative Stress In A Rat Model Of Parkinson’s Disease. Pharmacol Biochem Behav 138:96–103
pubmed: 26394281
doi: 10.1016/j.pbb.2015.09.013
Wang XF, Xing ML, Shen Y, Zhu X, Xu LH (2006) Oral Administration Of Cr(Vi) Induced Oxidative Stress, Dna Damage And Apoptotic Cell Death In Mice. Toxicology 228:16–23
pubmed: 16979809
doi: 10.1016/j.tox.2006.08.005
Wise JP Jr, Young JL, Cai J, Cai L (2022) Current Understanding Of Hexavalent Chromium [Cr(Vi)] Neurotoxicity And New Perspectives. Environ Int 158:106877
pubmed: 34547640
doi: 10.1016/j.envint.2021.106877
Witt KL, Stout MD, Herbert RA, Travlos GS, Kissling GE, Collins BJ, Hooth MJ (2013) Mechanistic Insights From The Ntp Studies Of Chromium. Toxicol Pathol 41:326–342
pubmed: 23334696
doi: 10.1177/0192623312469856
Yang X, Fang Y, Hou J, Wang X, Li J, Li S, Zheng X, Liu Y, Zhang Z (2022) The Heart As A Target For Deltamethrin Toxicity: Inhibition Of Nrf2/Ho-1 Pathway Induces Oxidative Stress And Results In Inflammation And Apoptosis. Chemosphere 300:134479
pubmed: 35367492
doi: 10.1016/j.chemosphere.2022.134479
Yang X, Zhang Y, Xu H, Luo X, Yu J, Liu J, Chang RC (2016) Neuroprotection Of Coenzyme Q10 In Neurodegenerative Diseases. Curr Top Med Chem 16:858–866
pubmed: 26311425
doi: 10.2174/1568026615666150827095252
Zendehdel R, Shetab-Boushehri SV, Azari MR, Hosseini V, Mohammadi H (2015) Chemometrics Models For Assessment Of Oxidative Stress Risk In Chrome-Electroplating Workers. Drug Chem Toxicol 38:174–179
pubmed: 24896654
doi: 10.3109/01480545.2014.922096