endothelin receptor type B
enteric nervous system
enteric neurons
hirschsprung disease
single-cell RNA sequencing
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2022
2022
Historique:
received:
10
04
2022
accepted:
06
07
2022
entrez:
12
8
2022
pubmed:
13
8
2022
medline:
13
8
2022
Statut:
epublish
Résumé
Hirschsprung disease is most often characterized by aganglionosis limited to the distal colon and rectum, and mice lacking the Endothelin receptor type B (Ednrb) faithfully recapitulate this phenotype. However, despite the presence of enteric ganglia in the small intestine, both human patients and Ednrb-/- mice suffer from dysmotility and altered gastrointestinal function, thus raising the possibility of enteric nervous system (ENS) abnormalities proximal to the aganglionic region. We undertook the present study to determine whether abnormalities with the ENS in ganglionated regions may account for abnormal gastrointestinal function. We performed single-cell RNA sequencing on ENS cells from the small intestine of Ednrb-/- mice and compared the results to a published single-cell dataset. Our results identified a missing population of neurons marked by the enzyme Gad2, which catalyzes the production of
Identifiants
pubmed: 35959491
doi: 10.3389/fcell.2022.917243
pii: 917243
pmc: PMC9360620
doi:
Types de publication
Journal Article
Langues
eng
Pagination
917243Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK119210
Pays : United States
Informations de copyright
Copyright © 2022 Bhave, Guyer, Picard, Omer, Hotta and Goldstein.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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