The mitochondrial enzyme FAHD1 regulates complex II activity in breast cancer cells and is indispensable for basal BT-20 cells in vitro.
FAHD1
MCF-7
TNBC
glutaminase
oxaloacetate
succinate dehydrogenase
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
revised:
29
06
2022
received:
09
05
2022
accepted:
30
06
2022
pubmed:
14
8
2022
medline:
18
11
2022
entrez:
13
8
2022
Statut:
ppublish
Résumé
The mitochondrial enzyme fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) was identified to be upregulated in breast cancer tissues. Here, we show that FAHD1 is indispensable for the survival of BT-20 cells, representing the basal breast cancer cell type. A lentiviral knock-down of FAHD1 in the breast cancer cell lines MCF-7 and BT-20 results in lower succinate dehydrogenase (complex II) activity. In luminal MCF-7 cells, this leads to reduced proliferation when cultured in medium containing only glutamine as the carbon source. Of note, both cell lines show attenuated protein levels of the enzyme glutaminase (GLS) which activates programmed cell death in BT-20. These findings demonstrate that FAHD1 is crucial for the functionality of complex II in breast cancer cells and acts on glutaminolysis in the mitochondria.
Identifiants
pubmed: 35962472
doi: 10.1002/1873-3468.14462
pmc: PMC7613834
mid: EMS153221
doi:
Substances chimiques
Glutamine
0RH81L854J
Hydrolases
EC 3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2781-2794Subventions
Organisme : Austrian Science Fund FWF
ID : P 31582
Pays : Austria
Informations de copyright
© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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