Transcriptional regulation by a RecQ helicase.
ChIP-seq
Genomic stability
Helicase
RNA-seq
RecQ
Transcription
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
14
8
2022
pubmed:
15
8
2022
medline:
17
8
2022
Statut:
ppublish
Résumé
RecQ helicases participate in a variety of DNA metabolic processes through their multiple biochemical activities. In vitro characterization and cellular studies have suggested that RECQ1 (also known as RECQL or RECQL1) performs its diverse functions through specific interactions with DNA and protein partners. We have taken an unbiased approach to determine the contribution of RECQ1 in genome maintenance and as a putative susceptibility factor in breast cancer. Here, we provide methodology to map the genome-wide binding sites of RECQ1 together with the profiling of RECQ1-dependent transcriptome to investigate its role in gene regulation. The described approach will be helpful to develop a mechanistic framework for elucidating critical functions of RECQ1 and other RecQ homologs in distinct chromatin and biological contexts.
Identifiants
pubmed: 35965009
pii: S0076-6879(22)00148-3
doi: 10.1016/bs.mie.2022.03.057
pmc: PMC9379128
mid: NIHMS1812400
pii:
doi:
Substances chimiques
DNA
9007-49-2
RecQ Helicases
EC 3.6.4.12
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-249Subventions
Organisme : NIGMS NIH HHS
ID : SC1 GM093999
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
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