Hydrolysis capacity of different sized granules in a full-scale aerobic granular sludge (AGS) reactor.

AGS, aerobic granular sludge AS, activated sludge Activity staining Aerobic granular sludge Biomass segregation COD, chemical oxygen demand EBPR, enhanced biological phosphorus removal EPS, extracellular polymeric substances FISH, fluorescence in situ hybridization GAO, glycogen-accumulating organism Hydrolysis PAO, polyphosphate-accumulating organism Polymeric substrates SBR, sequencing batch reactor SND, simultaneous nitrification-denitrification SRT, solids retention time TSS, total suspended solids VFA, volatile fatty acid VSS, volatile suspended solids WWTP, wastewater treatment plant Wastewater treatment

Journal

Water research X
ISSN: 2589-9147
Titre abrégé: Water Res X
Pays: England
ID NLM: 101742109

Informations de publication

Date de publication:
01 Aug 2022
Historique:
received: 17 05 2022
revised: 27 07 2022
accepted: 30 07 2022
entrez: 15 8 2022
pubmed: 16 8 2022
medline: 16 8 2022
Statut: epublish

Résumé

In aerobic granular sludge (AGS) reactors, granules of different sizes coexist in a single reactor. Their differences in settling behaviour cause stratification in the settled granule bed. In combination with substrate concentration gradients over the reactor height during the anaerobic plug-flow feeding regime, this can result in functional differences between granule sizes. In this study, we compared the hydrolytic activity in granules of 4 size ranges (between 0.5 and 4.8 mm diameter) collected from a full-scale AGS installation. Protease and amylase activities were quantified through fluorescent activity assays. To visualise where the hydrolytic active zones were located within the granules, the hydrolysis sites were visualized microscopically after incubating intact and sliced granules with fluorescent casein and starch. The microbial community was studied using fluorescent

Identifiants

pubmed: 35965888
doi: 10.1016/j.wroa.2022.100151
pii: S2589-9147(22)00021-4
pmc: PMC9364025
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100151

Informations de copyright

© 2022 The Authors. Published by Elsevier Ltd.

Déclaration de conflit d'intérêts

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Sara Toja Ortega (S)

Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Lenno van den Berg (L)

Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Mario Pronk (M)

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft, HZ 2629, the Netherlands.
Royal HaskoningDHV, Laan 1914 35, Amersfoort, AL 3800, the Netherlands.

Merle K de Kreuk (MK)

Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, Delft 2628CN, the Netherlands.

Classifications MeSH