Utility of serum indices in a particular case of serum protein electrophoresis.
capillary zone electrophoresis
interferences
serum indices
Journal
Biochemia medica
ISSN: 1846-7482
Titre abrégé: Biochem Med (Zagreb)
Pays: Croatia
ID NLM: 9610305
Informations de publication
Date de publication:
01 Oct 2022
01 Oct 2022
Historique:
received:
16
02
2022
accepted:
06
06
2022
entrez:
15
8
2022
pubmed:
16
8
2022
medline:
17
8
2022
Statut:
ppublish
Résumé
Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality.
Identifiants
pubmed: 35966258
doi: 10.11613/BM.2022.030802
pii: bm-32-3-030802
pmc: PMC9344864
doi:
Substances chimiques
Blood Proteins
0
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
030802Informations de copyright
Croatian Society of Medical Biochemistry and Laboratory Medicine.
Déclaration de conflit d'intérêts
Potential conflict of interest None declared.
Références
Expert Rev Mol Diagn. 2014 Jan;14(1):55-66
pubmed: 24308339
Clin Chem Lab Med. 2016 Jun 1;54(6):963-6
pubmed: 26574893
Blood Transfus. 2018 Sep;16(5):433-437
pubmed: 29757133
Biochem Med (Zagreb). 2021 Jun 15;31(2):020703
pubmed: 33927553
Clin Chim Acta. 2018 Sep;484:328-332
pubmed: 29617625
Ann Clin Biochem. 2016 Sep;53(Pt 5):527-38
pubmed: 27147624
Clin Chem Lab Med. 2016 Jun 1;54(6):947-61
pubmed: 26910744
Clin Biochem. 2013 Sep;46(13-14):1281-4
pubmed: 23792262
Clin Chem Lab Med. 2019 Dec 18;58(1):e1-e4
pubmed: 31343978
Clin Biochem. 2018 Jan;51:48-55
pubmed: 28965683
Biochem Med (Zagreb). 2014 Feb 15;24(1):80-8
pubmed: 24627717