The dynamic transcriptome during maturation of biofilms formed by methicillin-resistant
MRSA
Staphylococcus aureus
biofilm
clumping factor A
transcriptome
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2022
2022
Historique:
received:
23
02
2022
accepted:
04
07
2022
entrez:
15
8
2022
pubmed:
16
8
2022
medline:
16
8
2022
Statut:
epublish
Résumé
Methicillin-resistant Biofilm formation was assessed using a static model with one representative strain per clone. Total RNA was extracted from biofilm and planktonic cultures after 24, 48, and 72 h of growth, followed by rRNA depletion and sequencing (Illumina Inc., San Diego, CA, United States, NextSeq500, v2, 1 × 75 bp). Differentially expressed gene (DEG) analysis between phenotypes and among early (24 h), intermediate (48 h), and late (72 h) stages of biofilms was performed together with Genes involved in primarily core genome-encoded KEGG pathways (transporters and others) were upregulated in 24-h biofilm culture compared to 24-h planktonic culture. However, the number of affected pathways in the ST239 24 h biofilm ( Our data show striking differences in gene expression during biofilm formation by three of the most important pandemic MRSA clones, USA300, HEMRSA-15, and ST239. The
Sections du résumé
Background
UNASSIGNED
Methicillin-resistant
Methods
UNASSIGNED
Biofilm formation was assessed using a static model with one representative strain per clone. Total RNA was extracted from biofilm and planktonic cultures after 24, 48, and 72 h of growth, followed by rRNA depletion and sequencing (Illumina Inc., San Diego, CA, United States, NextSeq500, v2, 1 × 75 bp). Differentially expressed gene (DEG) analysis between phenotypes and among early (24 h), intermediate (48 h), and late (72 h) stages of biofilms was performed together with
Results
UNASSIGNED
Genes involved in primarily core genome-encoded KEGG pathways (transporters and others) were upregulated in 24-h biofilm culture compared to 24-h planktonic culture. However, the number of affected pathways in the ST239 24 h biofilm (
Conclusion
UNASSIGNED
Our data show striking differences in gene expression during biofilm formation by three of the most important pandemic MRSA clones, USA300, HEMRSA-15, and ST239. The
Identifiants
pubmed: 35966712
doi: 10.3389/fmicb.2022.882346
pmc: PMC9366926
doi:
Types de publication
Journal Article
Langues
eng
Pagination
882346Informations de copyright
Copyright © 2022 Vlaeminck, Lin, Xavier, De Backer, Berkell, De Greve, Hernalsteens, Kumar-Singh, Goossens and Malhotra-Kumar.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Microbiol Rev. 1986 Sep;50(3):314-52
pubmed: 3534538
Antimicrob Agents Chemother. 2021 Jun 17;65(7):e0183220
pubmed: 33903108
Proteomics. 2006 Mar;6(6):1867-77
pubmed: 16470655
Microbes Infect. 2000 Apr;2(5):533-42
pubmed: 10865198
mBio. 2013 Feb 12;4(1):e00537-12
pubmed: 23404398
FEMS Microbiol Rev. 2011 Sep;35(5):768-89
pubmed: 21645016
Infect Immun. 2007 Sep;75(9):4219-26
pubmed: 17576756
ChemMedChem. 2021 Jan 8;16(1):65-80
pubmed: 33090669
Nat Rev Microbiol. 2005 Sep;3(9):700-10
pubmed: 16138098
mBio. 2013 Dec 17;4(6):e00889-13
pubmed: 24345744
Appl Environ Microbiol. 2005 May;71(5):2663-76
pubmed: 15870358
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
J Infect Dis. 2016 Jan 1;213(1):139-48
pubmed: 26136471
Microbiology (Reading). 2007 Jul;153(Pt 7):2289-2303
pubmed: 17600073
Microb Genom. 2021 Jul;7(7):
pubmed: 34227933
Antimicrob Agents Chemother. 2007 Jan;51(1):264-74
pubmed: 17043114
Infect Genet Evol. 2013 Aug;18:106-12
pubmed: 23669446
J Bacteriol. 2004 Jul;186(14):4665-84
pubmed: 15231800
PLoS Pathog. 2012;8(4):e1002626
pubmed: 22496652
Microorganisms. 2018 Oct 29;6(4):
pubmed: 30380651
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5564-5569
pubmed: 29735708
J Bacteriol. 2001 Dec;183(24):7341-53
pubmed: 11717293
Infect Immun. 2001 Jun;69(6):4079-85
pubmed: 11349079
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5094-E5102
pubmed: 28607050
PLoS One. 2014 Aug 08;9(8):e104561
pubmed: 25105505
Antimicrob Agents Chemother. 2000 Jun;44(6):1549-55
pubmed: 10817707
Virulence. 2011 Sep-Oct;2(5):445-59
pubmed: 21921685
Nat Rev Dis Primers. 2018 May 31;4:18033
pubmed: 29849094
Front Microbiol. 2021 Oct 15;12:750489
pubmed: 34721354
J Biol Chem. 2016 Jun 10;291(24):12556-12564
pubmed: 27129223
Microbiol Mol Biol Rev. 2003 Sep;67(3):429-53, table of contents
pubmed: 12966143
J Clin Microbiol. 2007 May;45(5):1379-88
pubmed: 17329452
Antimicrob Agents Chemother. 1999 Jun;43(6):1449-58
pubmed: 10348769
mBio. 2020 Jul 7;11(4):
pubmed: 32636245
J Infect Dis. 2008 Jun 1;197(11):1523-30
pubmed: 18700257
J Bacteriol. 2007 Jun;189(11):4275-89
pubmed: 17384184
Int J Med Microbiol. 2013 Aug;303(6-7):324-30
pubmed: 23517691
Front Microbiol. 2016 Nov 15;7:1818
pubmed: 27895635
Medchemcomm. 2019 Mar 14;10(8):1231-1241
pubmed: 31534648
Science. 1999 May 21;284(5418):1318-22
pubmed: 10334980
Environ Microbiol. 2000 Apr;2(2):169-77
pubmed: 11220303
Microbiol Rev. 1995 Sep;59(3):451-80
pubmed: 7565414
J Med Microbiol. 2019 Mar;68(3):355-367
pubmed: 30628885
J Bacteriol. 2014 Jun;196(12):2277-89
pubmed: 24727224
Bioinformatics. 2014 Jul 15;30(14):2068-9
pubmed: 24642063