Transcriptional heterogeneity and cell cycle regulation as central determinants of Primitive Endoderm priming.
cell cycle
developmental biology
differentiation
embryonic stem cells
endoderm
heterogeneity
mouse
regenerative medicine
selection
stem cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
15 08 2022
15 08 2022
Historique:
received:
25
03
2022
accepted:
12
08
2022
pubmed:
16
8
2022
medline:
30
8
2022
entrez:
15
8
2022
Statut:
epublish
Résumé
During embryonic development cells acquire identity as they proliferate, implying that an intrinsic facet of cell fate choice requires coupling lineage decisions to cell division. How is the cell cycle regulated to promote or suppress heterogeneity and differentiation? We explore this question combining time lapse imaging with single-cell RNA-seq in the contexts of self-renewal, priming, and differentiation of mouse embryonic stem cells (ESCs) towards the Primitive Endoderm (PrE) lineage. Since ESCs are derived from the inner cell mass (ICM) of the mammalian blastocyst, ESCs in standard culture conditions are transcriptionally heterogeneous containing dynamically interconverting subfractions primed for either of the two ICM lineages, Epiblast and PrE. Here, we find that differential regulation of cell cycle can tip the balance between these primed populations, such that naïve ESC culture promotes Epiblast-like expansion and PrE differentiation stimulates the selective survival and proliferation of PrE-primed cells. In endoderm differentiation, this change is accompanied by a counter-intuitive increase in G1 length, also observed
Identifiants
pubmed: 35969041
doi: 10.7554/eLife.78967
pii: 78967
pmc: PMC9417417
doi:
pii:
Substances chimiques
Fibroblast Growth Factors
62031-54-3
Banques de données
GEO
['GSE200534', 'GSE123046']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, Perera et al.
Déclaration de conflit d'intérêts
MP, SN, MP, SP, RM, AT No competing interests declared, JB Reviewing editor, eLife
Références
Development. 2015 Aug 15;142(16):2840-9
pubmed: 26209649
Nature. 2019 Nov;575(7782):355-360
pubmed: 31695196
Sci Rep. 2019 May 29;9(1):8051
pubmed: 31142785
Development. 2007 Aug;134(16):2895-902
pubmed: 17660198
Dev Cell. 2006 May;10(5):615-24
pubmed: 16678776
Development. 2009 Oct;136(19):3215-22
pubmed: 19710168
Mol Cell. 2014 Jul 17;55(2):319-31
pubmed: 25038413
Science. 2014 Feb 14;343(6172):776-9
pubmed: 24531970
BMC Dev Biol. 2017 Jun 13;17(1):7
pubmed: 28610558
Elife. 2016 Oct 10;5:
pubmed: 27723457
PLoS Biol. 2015 Mar 18;13(3):e1002111
pubmed: 25786211
Annu Rev Cell Dev Biol. 2014;30:647-75
pubmed: 25288119
Dev Biol. 1987 May;121(1):10-9
pubmed: 2883052
Development. 1989 Apr;105(4):733-7
pubmed: 2598811
Nature. 2019 May;569(7756):361-367
pubmed: 30959515
Development. 2014 Jul;141(14):2770-9
pubmed: 25005472
Stem Cell Reports. 2018 Jul 10;11(1):58-69
pubmed: 29779897
Nat Cell Biol. 2017 Oct;19(10):1164-1177
pubmed: 28945231
Cell. 2008 Feb 8;132(3):487-98
pubmed: 18267078
J Cell Biol. 2018 May 7;217(5):1667-1685
pubmed: 29599208
Cell Rep. 2014 Dec 24;9(6):2056-70
pubmed: 25533345
Nat Cell Biol. 2022 Jun;24(6):833-844
pubmed: 35681011
Nature. 1981 Jul 9;292(5819):154-6
pubmed: 7242681
Cell Rep. 2013 Jun 27;3(6):1945-57
pubmed: 23746443
Development. 2010 Mar;137(5):715-24
pubmed: 20147376
Oncogene. 2009 Jan 8;28(1):20-30
pubmed: 18806832
Mol Syst Biol. 2019 Sep;15(9):e9002
pubmed: 31556488
Development. 2013 Jan 15;140(2):459-70
pubmed: 23193167
Nature. 2008 May 22;453(7194):519-23
pubmed: 18497825
Dev Dyn. 1998 May;212(1):49-62
pubmed: 9603423
Cell Stem Cell. 2009 Sep 4;5(3):320-31
pubmed: 19733543
Stem Cells Dev. 2013 Jan 15;22(2):279-95
pubmed: 22827698
Cell Differ Dev. 1990 Mar;29(3):181-6
pubmed: 2112419
Development. 2020 Aug 26;147(16):
pubmed: 32847824
Stem Cell Res. 2007 Oct;1(1):45-60
pubmed: 19383386
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8
pubmed: 6950406
Stem Cell Res. 2013 Jan;10(1):118-31
pubmed: 23178806
Cell. 2013 Sep 26;155(1):135-47
pubmed: 24074866
Development. 1990 Dec;110(4):1241-8
pubmed: 2100261
Nat Commun. 2016 Nov 18;7:13463
pubmed: 27857135
Nature. 2015 Mar 26;519(7544):468-71
pubmed: 25762143
Nat Cell Biol. 2015 Oct;17(10):1235-46
pubmed: 26389663
Elife. 2022 Aug 15;11:
pubmed: 35969041
Dev Cell. 2017 Apr 24;41(2):129-142.e6
pubmed: 28441528
Curr Biol. 2006 Jun 20;16(12):1171-82
pubmed: 16782007
Cell Stem Cell. 2017 Oct 5;21(4):449-455.e4
pubmed: 28985526
PLoS Biol. 2010 May 25;8(5):e1000379
pubmed: 20520791