LEDGF is a new growth factor in fetal serum.

Animals Epidermal Growth Factor / pharmacology Insulins Intercellular Signaling Peptides and Proteins Mammals / metabolism Tandem Mass Spectrometry Transcription Factors / genetics
Adult serum Fetal serum Growth factors LC-ESI-MS/MS Lens epithelium derived growth factor (LEDGF/PSIP1) Nano electrospray ionization tandem mass spectrometry Organic extraction Quaternary amine chromatography Random and independent sampling SQL SERVER & R

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 10 2022
Historique:
received: 26 02 2022
revised: 23 06 2022
accepted: 01 08 2022
pubmed: 16 8 2022
medline: 14 9 2022
entrez: 15 8 2022
Statut: ppublish

Résumé

Fetal serum supports the immortal growth of mammalian cell lines in culture while adult serum leads to the terminal differentiation and death of cells in culture. Many of the proteins in fetal serum that support the indefinite division and growth of cancerous cell lines remain obscure. The peptides and proteins of fetal versus adult serum were analyzed by liquid chromatography, nano electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS). Three batches of fetal serum contained the Alpha Fetoprotein marker while adult serum batches did not. Insulin (INS), and insulin-like growth factor (ILGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and platelet derived growth factor (PDGF) were increased in fetal serum. New fetal growth factors including MEGF, HDGFRP and PSIP1 and soluble growth receptors such as TNFR, EGFR, NTRK2 and THRA were discovered. Addition of insulin or the homeotic transcription factor PSIP1, also referred to as Lens Epithelium Derived Growth Factor (LEDGF), partially restored the rounded phenotype of rapidly dividing cells but was not as effective as fetal serum. Thus, a new growth factor in fetal serum, LEDGF/PSIP1, was directly observed by tandem mass spectrometry and confirmed by add back experiments to cell culture media alongside insulin.

Identifiants

DOI: 10.1016/j.ab.2022.114845 PMID: 35970411
pubmed: 35970411
pii: S0003-2697(22)00305-0
doi: 10.1016/j.ab.2022.114845
pii:
doi:

Substances chimiques

Insulins 0
Intercellular Signaling Peptides and Proteins 0
Transcription Factors 0
lens epithelium-derived growth factor 0
Epidermal Growth Factor 62229-50-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114845

Informations de copyright

Copyright © 2022. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no competing interests.

Auteurs

Zhuo Zhen Chen (ZZ)

Research Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address: zzchen@ryerson.ca.

Peter Bowden (P)

Research Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address: pbowden@start.ca.

Jaimie Dufresne (J)

Research Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address: jaimie.dufresne@ryerson.ca.

Ming Miao (M)

Research Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address: ming.miao@yyzpharmatech.com.

John G Marshall (JG)

Research Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address: 4marshal@ryerson.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la famille de l'EGF › Facteur de croissance épidermique › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Insulines › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › LEDGF is a new growth factor in fetal serum.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › LEDGF is a new growth factor in fetal serum.