Endothelial-Smooth Muscle Cell Interactions in a Shear-Exposed Intimal Hyperplasia on-a-Dish Model to Evaluate Therapeutic Strategies.
drug screening
endothelial cell networks
flow
in vitro coculture model
vascular injury
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
21
04
2022
pubmed:
16
8
2022
medline:
12
10
2022
entrez:
15
8
2022
Statut:
ppublish
Résumé
Intimal hyperplasia (IH) represents a major challenge following cardiovascular interventions. While mechanisms are poorly understood, the inefficient preventive methods incentivize the search for novel therapies. A vessel-on-a-dish platform is presented, consisting of direct-contact cocultures with human primary endothelial cells (ECs) and smooth muscle cells (SMCs) exposed to both laminar pulsatile and disturbed flow on an orbital shaker. With contractile SMCs sitting below a confluent EC layer, a model that successfully replicates the architecture of a quiescent vessel wall is created. In the novel IH model, ECs are seeded on synthetic SMCs at low density, mimicking reendothelization after vascular injury. Over 3 days of coculture, ECs transition from a network conformation to confluent 2D islands, as promoted by pulsatile flow, resulting in a "defected" EC monolayer. In defected regions, SMCs incorporated plasma fibronectin into fibers, increased proliferation, and formed multilayers, similarly to IH in vivo. These phenomena are inhibited under confluent EC layers, supporting therapeutic approaches that focus on endothelial regeneration rather than inhibiting proliferation, as illustrated in a proof-of-concept experiment with Paclitaxel. Thus, this in vitro system offers a new tool to study EC-SMC communication in IH pathophysiology, while providing an easy-to-use translational disease model platform for low-cost and high-content therapeutic development.
Identifiants
pubmed: 35971167
doi: 10.1002/advs.202202317
pmc: PMC9534971
doi:
Substances chimiques
Fibronectins
0
Paclitaxel
P88XT4IS4D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2202317Subventions
Organisme : Zurich Heart
Organisme : ETHeart Initiative
Organisme : ETH Zurich
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Circ Res. 2007 Apr 13;100(7):1055-62
pubmed: 17347479
J Vasc Surg. 2005 May;41(5):869-80
pubmed: 15886673
Circulation. 1995 Jun 1;91(11):2793-801
pubmed: 7758186
PLoS One. 2009 Jun 04;4(6):e5798
pubmed: 19495422
J Biomech. 2004 Apr;37(4):531-9
pubmed: 14996565
J R Soc Interface. 2013 Oct 23;11(90):20130852
pubmed: 24152813
Ann Biomed Eng. 2011 Nov;39(11):2750-8
pubmed: 21811870
Vascul Pharmacol. 2015 Sep;72:9-15
pubmed: 26093336
J Surg Res. 2003 Jan;109(1):16-23
pubmed: 12591230
Atherosclerosis. 2001 Feb 1;154(2):449-53
pubmed: 11166778
Biotechnol Bioeng. 2016 Jun;113(6):1336-44
pubmed: 26615057
Biomaterials. 2005 Aug;26(22):4642-53
pubmed: 15722134
Circ Res. 1999 Mar 5;84(4):384-91
pubmed: 10066672
PLoS One. 2010 Aug 16;5(8):e12196
pubmed: 20808940
Biomaterials. 2001 Mar;22(5):439-44
pubmed: 11214754
Annu Rev Physiol. 2018 Feb 10;80:353-387
pubmed: 29433414
Vasc Med. 2000;5(1):41-8
pubmed: 10737155
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1908-13
pubmed: 21245329
Int J Mol Sci. 2021 Dec 28;23(1):
pubmed: 35008757
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13379-84
pubmed: 25139991
Cardiol Rev. 2013 Mar-Apr;21(2):101-9
pubmed: 22968180
Exp Cell Res. 1992 Jun;200(2):358-60
pubmed: 1572403
Nat Rev Mol Cell Biol. 2004 Apr;5(4):261-70
pubmed: 15071551
Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1978-86
pubmed: 17644568
PLoS Comput Biol. 2014 Aug 14;10(8):e1003774
pubmed: 25121971
Fibrogenesis Tissue Repair. 2011 Sep 16;4:21
pubmed: 21923916
Cell Cycle. 2017 Oct 18;16(20):1893-1901
pubmed: 28820314
Tissue Eng Part A. 2010 Jun;16(6):1901-12
pubmed: 20088699
Comput Methods Biomech Biomed Engin. 2016;19(6):581-90
pubmed: 26096592
Wound Repair Regen. 2008 Mar-Apr;16(2):300-9
pubmed: 18318814
Ther Adv Cardiovasc Dis. 2010 Apr;4(2):129-41
pubmed: 20200200
Arterioscler Thromb Vasc Biol. 2006 Apr;26(4):780-6
pubmed: 16469949
Eur Heart J. 2020 Jan 7;41(2):200-203
pubmed: 31909425
Nat Rev Cardiol. 2020 Jan;17(1):37-51
pubmed: 31346257
Nat Rev Cardiol. 2021 Aug;18(8):565-580
pubmed: 33627876
Mol Cancer Ther. 2004 Oct;3(10):1301-10
pubmed: 15486197
Nat Commun. 2015 Aug 14;6:8026
pubmed: 26272817
Biochim Biophys Acta. 2016 Jul;1863(7 Pt A):1519-33
pubmed: 27012600
Ann Biomed Eng. 2011 Jun;39(6):1608-19
pubmed: 21479754
Tissue Eng Part C Methods. 2017 Jan;23(1):38-49
pubmed: 27923320
J Invest Dermatol. 2002 Jul;119(1):143-53
pubmed: 12164937
Circulation. 2004 Jul 6;110(1):36-45
pubmed: 15210598
Atherosclerosis. 2019 Jun;285:170-177
pubmed: 31096159
Lancet. 2004 Oct 23-29;364(9444):1519-21
pubmed: 15500897
Prog Biomater. 2018 Sep;7(3):175-205
pubmed: 30203125
J Vasc Surg. 2005 Mar;41(3):509-16
pubmed: 15838487
Nat Rev Cardiol. 2016 Aug;13(8):451-70
pubmed: 27194091
Circulation. 1997 Jul 15;96(2):636-45
pubmed: 9244237
J Magn Reson Imaging. 1995 Nov-Dec;5(6):640-7
pubmed: 8748480
Acta Biomater. 2019 Nov;99:53-71
pubmed: 31454565
World J Cardiol. 2017 Aug 26;9(8):640-651
pubmed: 28932353
Semin Immunopathol. 2012 Jan;34(1):93-106
pubmed: 21845431
Rev Port Cir Cardiotorac Vasc. 2019 Jul-Sep;26(3):213-217
pubmed: 31734974
Expert Rev Med Devices. 2017 May;14(5):383-392
pubmed: 28447487
Adv Sci (Weinh). 2022 Oct;9(28):e2202317
pubmed: 35971167
Cytoskeleton (Hoboken). 2015 Jun;72(6):257-67
pubmed: 26147585
Coron Artery Dis. 2003 May;14(3):219-24
pubmed: 12702925
Arq Bras Cardiol. 2014 Sep;103(3):174-82
pubmed: 25098375
PLoS One. 2022 Jan 21;17(1):e0262044
pubmed: 35061745
Circ Res. 2003 Jul 25;93(2):e17-24
pubmed: 12829619
Front Cardiovasc Med. 2018 Oct 23;5:151
pubmed: 30406116
Ann Biomed Eng. 2007 Mar;35(3):375-86
pubmed: 17191127
Arterioscler Thromb Vasc Biol. 2003 Jun 1;23(6):1008-13
pubmed: 12676801
J Vasc Surg. 2005 Feb;41(2):321-31
pubmed: 15768016
J R Soc Interface. 2014 Jul 6;11(96):
pubmed: 24829281
Nat Rev Cardiol. 2020 Mar;17(3):155-169
pubmed: 31455868
Atherosclerosis. 2017 Oct;265:266-274
pubmed: 28865843
PLoS One. 2016 Apr 08;11(4):e0150806
pubmed: 27058862
Exp Cell Res. 2013 Oct 1;319(16):2409-17
pubmed: 23800466
J Cell Biol. 2006 Jan 16;172(2):259-68
pubmed: 16401722
JVS Vasc Sci. 2021 May 15;2:79-94
pubmed: 34617061
Physiology (Bethesda). 2014 Jul;29(4):234-41
pubmed: 24985327
Nat Commun. 2015 Jun 04;6:7275
pubmed: 26041410
Biomech Model Mechanobiol. 2017 Feb;16(1):197-211
pubmed: 27456489
Ann Biomed Eng. 2012 Mar;40(3):707-28
pubmed: 22042624
Thromb Haemost. 2007 Mar;97(3):355-63
pubmed: 17334501
Cell Biochem Biophys. 2008;52(1):37-46
pubmed: 18766304
Ann Biomed Eng. 2010 Mar;38(3):801-12
pubmed: 20033777
Gene. 2016 Jul 1;585(1):143-153
pubmed: 26970176
Adv Healthc Mater. 2020 Apr;9(8):e1901255
pubmed: 32100473
J Am Heart Assoc. 2018 Dec 18;7(24):e011245
pubmed: 30561254
Tissue Eng. 2006 Feb;12(2):381-90
pubmed: 16548696
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:3343-5
pubmed: 19964074
Angiogenesis. 2013 Jul;16(3):481-92
pubmed: 23389639
Adv Exp Med Biol. 2015;887:53-77
pubmed: 26662986
J Cardiovasc Transl Res. 2016 Apr;9(2):91-101
pubmed: 26797874
J Tissue Eng Regen Med. 2018 Nov;12(11):2164-2178
pubmed: 30079631
Sci Transl Med. 2018 May 9;10(440):
pubmed: 29743347
Matrix Biol. 2017 Jul;60-61:1-4
pubmed: 28527902
Nature. 2005 Sep 15;437(7057):426-31
pubmed: 16163360
J Pathol. 2000 Feb;190(3):300-9
pubmed: 10685064
Am J Physiol Heart Circ Physiol. 2010 Aug;299(2):H338-46
pubmed: 20495148
Int J Exp Pathol. 2000 Jun;81(3):173-82
pubmed: 10971738
Biomaterials. 2007 Sep;28(27):3965-76
pubmed: 17582491
J Am Coll Cardiol. 2010 Nov 30;56(23):1897-907
pubmed: 21109112
Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1137-41
pubmed: 7532305
Tissue Eng Part B Rev. 2010 Oct;16(5):467-91
pubmed: 20334504
Expert Rev Cardiovasc Ther. 2010 Apr;8(4):545-56
pubmed: 20397828
Arterioscler Thromb Vasc Biol. 2013 Sep;33(9):2130-6
pubmed: 23814115
Cardiovasc Res. 2013 Jul 15;99(2):269-75
pubmed: 23592806