Sewage surveillance of SARS-CoV-2 at student campus residences in the Western Cape, South Africa.

COVID-19 Institution Near-source WBE Passive sampling SARS-CoV-2 Student campus residences Wastewater Wastewater-based epidemiology

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
10 Dec 2022
Historique:
received: 28 03 2022
revised: 15 07 2022
accepted: 10 08 2022
pubmed: 17 8 2022
medline: 20 10 2022
entrez: 16 8 2022
Statut: ppublish

Résumé

The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic capacity is limited in defined communities, posing a challenge in tracking and tracing new infections. Monitoring student residences, which are considered infection hotspots, with targeted wastewater surveillance is crucial. This study evaluated the efficacy of SARS-CoV-2 targeted wastewater surveillance for outbreak mitigation at Stellenbosch University's student residences in South Africa. Using torpedo-style passive sampling devices, wastewater samples were collected biweekly from manholes at twelve Stellenbosch University Tygerberg (SUT) campus and Stellenbosch University-Main (SUM) campus student residences. The surveillance led to an early warning detection of SARS-CoV-2 presence on campus, followed by an informed management strategy leading to restriction of student activities on campus and a delay in the onset of the third wave that was experienced throughout the country. Moreover, the study highlighted the extent of possible infections at defined locations even when a low number of confirmed coronavirus disease 2019 (COVID-19) cases were reported. The study also tracked the surge of the Delta and Omicron variants in the student residences using the Thermo Fisher TaqMan® RT-qPCR genotyping assay.

Identifiants

pubmed: 35973539
pii: S0048-9697(22)05127-0
doi: 10.1016/j.scitotenv.2022.158028
pmc: PMC9375247
pii:
doi:

Substances chimiques

Sewage 0
Waste Water 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

158028

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Noluxabiso Mangwana (N)

Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, Cape Town 7505, South Africa; Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa.

Edward Archer (E)

Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa.

Christo J F Muller (CJF)

Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, Cape Town 7505, South Africa; Division of Medical Physiology, Faculty of Medicine and Health Sciences, Centre for Cardiometabolic Research in Africa, Stellenbosch University, South Africa.

Wolfgang Preiser (W)

Division of Medical Virology, Faculty of Medicine & Health Sciences, Stellenbosch University, Tygerberg, Cape Town 7505, South Africa; National Health Laboratory Services, Tygerberg Hospital, Tygerberg, Cape Town 7505, South Africa.

Gideon Wolfaardt (G)

Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa; Department of Chemistry and Biology, Ryerson University, Toronto, ON M5B 2K3, Canada.

Barbara Kasprzyk-Hordern (B)

Department of Chemistry, Faculty of Science, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom.

Alno Carstens (A)

Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa.

Ludwig Brocker (L)

Department of Microbiology, Stellenbosch University, Stellenbosch 7600, South Africa.

Candice Webster (C)

Environment and Health Research Unit, South African Medical Research Council (SAMRC), Johannesburg, South Africa.

David McCarthy (D)

Environmental and Public Health Microbiology Lab (EPHM LAB), Monash Infrastructure Institute, Department of Civil Engineering, Monash University, Clayton 3800, Australia.

Renee Street (R)

Environment and Health Research Unit, South African Medical Research Council (SAMRC), Durban, South Africa.

Angela Mathee (A)

Environment and Health Research Unit, South African Medical Research Council (SAMRC), Johannesburg, South Africa.

Johan Louw (J)

Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, Cape Town 7505, South Africa.

Mongezi Mdhluli (M)

Chief Research Operations Office, South African Medical Research Council, Tygerberg 7050, South Africa.

Rabia Johnson (R)

Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, Cape Town 7505, South Africa; Division of Medical Physiology, Faculty of Medicine and Health Sciences, Centre for Cardiometabolic Research in Africa, Stellenbosch University, South Africa. Electronic address: rabia.johnson@mrc.ac.za.

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