Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery.
bariatric surgery
immunometabolism
inflammation
influenza virus
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
28 12 2022
28 12 2022
Historique:
received:
25
05
2022
accepted:
15
08
2022
pubmed:
18
8
2022
medline:
31
12
2022
entrez:
17
8
2022
Statut:
ppublish
Résumé
Obesity dysregulates immunity to influenza infection. Therefore, there is a critical need to investigate how obesity impairs immunity and to establish therapeutic approaches that mitigate the impact of increased adiposity. One mechanism by which obesity may alter immune responses is through changes in cellular metabolism. We studied inflammation and cellular metabolism of peripheral blood mononuclear cells (PBMCs) isolated from individuals with obesity relative to lean controls. We also investigated if impairments to PBMC metabolism were reversible upon short-term weight loss following bariatric surgery. Obesity was associated with systemic inflammation and poor inflammation resolution. Unstimulated PBMCs from participants with obesity had lower oxidative metabolism and adenosine triphosphate (ATP) production compared to PBMCs from lean controls. PBMC secretome analyses showed that ex vivo stimulation with A/Cal/7/2009 H1N1 influenza led to a notable increase in IL-6 with obesity. Short-term weight loss via bariatric surgery improved biomarkers of systemic metabolism but did not improve markers of inflammation resolution, PBMC metabolism, or the PBMC secretome. These results show that obesity drives a signature of impaired PBMC metabolism, which may be due to persistent inflammation. PBMC metabolism was not reversed after short-term weight loss despite improvements in measures of systemic metabolism.
Sections du résumé
BACKGROUND
Obesity dysregulates immunity to influenza infection. Therefore, there is a critical need to investigate how obesity impairs immunity and to establish therapeutic approaches that mitigate the impact of increased adiposity. One mechanism by which obesity may alter immune responses is through changes in cellular metabolism.
METHODS
We studied inflammation and cellular metabolism of peripheral blood mononuclear cells (PBMCs) isolated from individuals with obesity relative to lean controls. We also investigated if impairments to PBMC metabolism were reversible upon short-term weight loss following bariatric surgery.
RESULTS
Obesity was associated with systemic inflammation and poor inflammation resolution. Unstimulated PBMCs from participants with obesity had lower oxidative metabolism and adenosine triphosphate (ATP) production compared to PBMCs from lean controls. PBMC secretome analyses showed that ex vivo stimulation with A/Cal/7/2009 H1N1 influenza led to a notable increase in IL-6 with obesity. Short-term weight loss via bariatric surgery improved biomarkers of systemic metabolism but did not improve markers of inflammation resolution, PBMC metabolism, or the PBMC secretome.
CONCLUSIONS
These results show that obesity drives a signature of impaired PBMC metabolism, which may be due to persistent inflammation. PBMC metabolism was not reversed after short-term weight loss despite improvements in measures of systemic metabolism.
Identifiants
pubmed: 35975968
pii: 6670556
doi: 10.1093/infdis/jiac345
pmc: PMC10205606
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
92-102Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK056350
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI159308
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002489
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA197627
Pays : United States
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Déclaration de conflit d'intérêts
Potential conflicts of interest. The authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
Références
PLoS One. 2015 Mar 17;10(3):e0121971
pubmed: 25781614
Surg Obes Relat Dis. 2011 Sep-Oct;7(5):618-24
pubmed: 21546319
J Infect Dis. 2019 Apr 19;219(10):1652-1661
pubmed: 30535161
Prostaglandins Other Lipid Mediat. 2020 Dec;151:106483
pubmed: 32998074
Arterioscler Thromb Vasc Biol. 2014 Dec;34(12):2637-43
pubmed: 25341798
J Leukoc Biol. 2022 Jan;111(1):147-159
pubmed: 33847405
Wideochir Inne Tech Maloinwazyjne. 2012 Dec;7(4):225-32
pubmed: 23362420
Surg Endosc. 2013 Dec;27(12):4569-73
pubmed: 23982645
Mol Metab. 2017 Feb 16;6(4):317-326
pubmed: 28377871
Diabetes. 2012 Feb;61(2):401-8
pubmed: 22228716
Surg Obes Relat Dis. 2017 Oct;13(10):1658-1663
pubmed: 28843985
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5133-8
pubmed: 23479618
Surg Endosc. 2009 May;23(5):930-49
pubmed: 19125308
Ann Surg. 2004 Sep;240(3):416-23; discussion 423-4
pubmed: 15319713
Obesity (Silver Spring). 2013 Nov;21(11):2377-86
pubmed: 23512822
Int J Obes (Lond). 2012 Aug;36(8):1072-7
pubmed: 22024641
Surg Obes Relat Dis. 2017 Feb;13(2):181-188
pubmed: 27692906
PLoS One. 2018 Jun 1;13(6):e0198214
pubmed: 29856816
Mol Aspects Med. 2018 Dec;64:1-17
pubmed: 28802833
Clin Infect Dis. 2011 Feb 1;52(3):301-12
pubmed: 21208911
Infect Immun. 2011 Nov;79(11):4668-73
pubmed: 21896775
Curr Opin Clin Nutr Metab Care. 2011 Jul;14(4):396-401
pubmed: 21505331
Obes Surg. 2013 Aug;23(8):1252-61
pubmed: 23475776
Cell Metab. 2019 Sep 3;30(3):447-461.e5
pubmed: 31378464
Obes Surg. 2011 Jul;21(7):902-9
pubmed: 21347823
Sci Rep. 2017 Aug 21;7(1):9007
pubmed: 28827671
Obes Surg. 2014 Jun;24(6):903-8
pubmed: 24566661
Int J Obes (Lond). 2017 Sep;41(9):1324-1330
pubmed: 28584297
J Clin Invest. 2018 Jul 2;128(7):2657-2669
pubmed: 29757195
Obes Rev. 2020 Nov;21(11):e13128
pubmed: 32845580
Hum Vaccin Immunother. 2014;10(5):1181-6
pubmed: 24614530
Methods Mol Biol. 2012;844:15-30
pubmed: 22262432
Sci Rep. 2016 May 31;6:26996
pubmed: 27243383
PLoS One. 2010 Mar 15;5(3):e9694
pubmed: 20300571