Cilia regulate meiotic recombination in zebrafish.
kif3a
cilia
homologous recombination
meiosis
zebrafish
Journal
Journal of molecular cell biology
ISSN: 1759-4685
Titre abrégé: J Mol Cell Biol
Pays: United States
ID NLM: 101503669
Informations de publication
Date de publication:
27 09 2022
27 09 2022
Historique:
received:
08
06
2022
revised:
16
06
2022
accepted:
15
08
2022
pubmed:
19
8
2022
medline:
22
12
2022
entrez:
18
8
2022
Statut:
ppublish
Résumé
Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside of the cell can regulate the meiosis process. Cilia are microtubule-based structures that protrude from the cell surface and function as signaling hubs to sense extracellular signals. Here, we reported an unexpected and critical role of cilia during meiotic recombination. During gametogenesis of zebrafish, cilia were specifically present in the prophase stages of both primary spermatocytes and primary oocytes. By developing a germ cell-specific CRISPR/Cas9 system, we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double-strand break repair, reduced crossover formation, and increased germ cell apoptosis. Our study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle.
Identifiants
pubmed: 35981808
pii: 6671532
doi: 10.1093/jmcb/mjac049
pmc: PMC9764210
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) (2022). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, CEMCS, CAS.
Références
Nat Rev Genet. 2019 Apr;20(4):221-234
pubmed: 30573849
Science. 2006 Aug 4;313(5787):629-33
pubmed: 16888132
Sci China Life Sci. 2021 Aug;64(8):1236-1280
pubmed: 33893979
Nature. 2000 Oct 19;407(6806):913-6
pubmed: 11057671
Cell. 1991 Mar 22;64(6):1155-61
pubmed: 2004421
Nat Commun. 2014 Jun 20;5:4207
pubmed: 24947469
PLoS Genet. 2020 Mar 20;16(3):e1008655
pubmed: 32196499
Cell. 2008 Aug 8;134(3):427-38
pubmed: 18692466
Development. 2006 Nov;133(21):4131-43
pubmed: 17021045
Nat Rev Nephrol. 2019 Apr;15(4):199-219
pubmed: 30733609
Neuron. 2004 Jun 10;42(5):703-16
pubmed: 15182712
Mar Biotechnol (NY). 2013 Oct;15(5):526-39
pubmed: 23535913
Cell. 2015 Nov 19;163(5):1252-1266
pubmed: 26548954
Cell. 1992 May 1;69(3):439-56
pubmed: 1581960
Cell Rep. 2013 Oct 17;5(1):37-43
pubmed: 24095732
J Genet Genomics. 2016 Mar 20;43(3):107-20
pubmed: 27020031
Annu Rev Cell Dev Biol. 2004;20:525-58
pubmed: 15473851
Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2388-93
pubmed: 22308397
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):
pubmed: 34088835
Development. 2006 Jul;133(14):2757-70
pubmed: 16775002
J Biol Chem. 2017 Oct 20;292(42):17375-17386
pubmed: 28855254
Nat Rev Mol Cell Biol. 2011 Apr;12(4):222-34
pubmed: 21427764
J Genet Genomics. 2020 Jan 20;47(1):37-47
pubmed: 32094061
Cold Spring Harb Perspect Biol. 2015 May 18;7(6):
pubmed: 25986558
Nat Rev Mol Cell Biol. 2001 Aug;2(8):621-7
pubmed: 11483995
Nat Cell Biol. 2022 Apr;24(4):483-496
pubmed: 35411088
Nature. 2005 Aug 18;436(7053):1053-7
pubmed: 16107854
Curr Biol. 2005 Aug 23;15(16):R633-5
pubmed: 16111936
Trends Genet. 2019 May;35(5):359-370
pubmed: 30948240
N Engl J Med. 2011 Apr 21;364(16):1533-43
pubmed: 21506742
Dev Cell. 2008 Aug;15(2):187-97
pubmed: 18694559
Nat Cell Biol. 2014 Feb;16(2):145-56
pubmed: 24413433
Cell. 1997 Feb 7;88(3):375-84
pubmed: 9039264
Cold Spring Harb Perspect Biol. 2015 Oct 28;7(12):
pubmed: 26511629
Nat Cell Biol. 2014 Jul;16(7):663-72
pubmed: 24952464
Curr Opin Genet Dev. 2013 Apr;23(2):147-55
pubmed: 23313097
PLoS Biol. 2016 Jan 07;14(1):e1002335
pubmed: 26741740
Cell. 2009 Nov 25;139(5):907-19
pubmed: 19913287
Nat Rev Genet. 2010 Feb;11(2):124-36
pubmed: 20051984
Curr Opin Cell Biol. 2018 Jun;52:22-29
pubmed: 29414590
Science. 2022 Jun 17;376(6599):eabh3104
pubmed: 35549308
Development. 2009 Sep;136(18):3089-98
pubmed: 19700616
Annu Rev Genet. 2016 Nov 23;50:293-316
pubmed: 27686280
Cell. 1994 Dec 16;79(6):1081-92
pubmed: 7528104
PLoS Genet. 2019 Jan 17;15(1):e1007730
pubmed: 30653507
Cell. 1992 May 1;69(3):457-70
pubmed: 1581961
Nat Rev Mol Cell Biol. 2017 Sep;18(9):533-547
pubmed: 28698599
Methods Cell Biol. 2016;135:3-17
pubmed: 27443918