Hemopexin as a Potential Binding Partner of Arginine-Rich Cell-Penetrating Peptides in Serum.
Journal
ACS pharmacology & translational science
ISSN: 2575-9108
Titre abrégé: ACS Pharmacol Transl Sci
Pays: United States
ID NLM: 101721411
Informations de publication
Date de publication:
12 Aug 2022
12 Aug 2022
Historique:
received:
10
03
2022
entrez:
19
8
2022
pubmed:
20
8
2022
medline:
20
8
2022
Statut:
epublish
Résumé
Serum proteins affect the in vivo fate and cellular uptake of arginine-rich cell-penetrating peptides (CPPs) and drugs delivered by CPPs. Although the binding of CPPs to serum proteins may possibly reduce their cellular uptake to some extent, it may also prolong their circulation half-life in vivo. We aimed to identify novel binding proteins of arginine-rich CPPs in serum to better understand their in vivo fate and develop more sophisticated drug delivery systems using CPPs. Isothermal titration calorimetry analysis suggests that albumin, the most abundant protein in serum, binds to d-forms of oligoarginine; however, the dissociation constants are several tens of a micromolar. Candidate proteins with the potential of binding to arginine-rich CPPs in serum were then explored using nondenaturing polyacrylamide gel electrophoresis followed by mass spectrometry analysis. Studies using fluorescence correlation spectroscopy determined hemopexin as a potential binding partner of d-forms of arginine-rich CPPs, including d-octaarginine (
Identifiants
pubmed: 35983275
doi: 10.1021/acsptsci.2c00043
pmc: PMC9379940
doi:
Types de publication
Journal Article
Langues
eng
Pagination
603-615Informations de copyright
© 2022 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare the following competing financial interest(s): This study was funded by Taiho Pharmaceutical Co., Ltd.
Références
Bioconjug Chem. 2016 Apr 20;27(4):1119-30
pubmed: 27019270
Biochim Biophys Acta. 2013 Dec;1830(12):5435-43
pubmed: 23665585
Nat Rev Nephrol. 2019 Nov;15(11):671-692
pubmed: 31455889
Chem Pharm Bull (Tokyo). 2021;69(7):601-607
pubmed: 34193708
J Biophys. 2011;2011:414729
pubmed: 21687343
Biochim Biophys Acta. 2013 Dec;1830(12):5526-34
pubmed: 23639804
Nat Nanotechnol. 2021 Jul;16(7):830-839
pubmed: 33958764
J Proteome Res. 2019 Jan 4;18(1):120-129
pubmed: 30480452
J Pharm Sci. 2019 Nov;108(11):3592-3598
pubmed: 31288036
Adv Drug Deliv Rev. 2005 Feb 28;57(4):547-58
pubmed: 15722163
Biophys J. 1995 Dec;69(6):2298-303
pubmed: 8599637
Chembiochem. 2018 Mar 2;19(5):430-433
pubmed: 29235220
J Biol Chem. 2001 Feb 23;276(8):5836-40
pubmed: 11084031
Clin Chim Acta. 2001 Oct;312(1-2):13-23
pubmed: 11580905
Nat Chem Biol. 2009 Nov;5(11):835-41
pubmed: 19749743
IUBMB Life. 2005 Nov;57(11):749-59
pubmed: 16511968
Philos Trans R Soc Lond B Biol Sci. 2019 Feb 4;374(1765):20180156
pubmed: 30967005
Mol Pharm. 2014 Aug 4;11(8):2553-65
pubmed: 24798708
J Proteome Res. 2008 Sep;7(9):3708-17
pubmed: 18707158
J Am Chem Soc. 2015 Feb 25;137(7):2536-2541
pubmed: 25679876
Front Oncol. 2021 Jan 19;10:570108
pubmed: 33542897
J Biol Chem. 1997 Jun 20;272(25):16010-7
pubmed: 9188504
ACS Chem Biol. 2016 Feb 19;11(2):400-8
pubmed: 26569370
J Biol Chem. 2008 Aug 22;283(34):23450-61
pubmed: 18550548
Mol Pharm. 2022 Feb 7;19(2):558-567
pubmed: 34958576
Antioxid Redox Signal. 2010 Feb;12(2):305-20
pubmed: 19650691
Methods. 2003 Feb;29(2):153-66
pubmed: 12606221
Mol Cell Ther. 2016 Feb 27;4:3
pubmed: 26925240
FASEB J. 2006 Sep;20(11):1775-84
pubmed: 16940149
Bioconjug Chem. 2008 Mar;19(3):656-64
pubmed: 18269225
Nat Med. 2000 Nov;6(11):1253-7
pubmed: 11062537
J Fluoresc. 2008 Nov;18(6):1093-101
pubmed: 18431548
Mol Ther. 2004 Dec;10(6):1011-22
pubmed: 15564133
J Biol Chem. 1994 Apr 8;269(14):10444-50
pubmed: 8144628
Chem Pharm Bull (Tokyo). 2020;68(7):583-588
pubmed: 32611995
FEBS Lett. 2013 Jun 19;587(12):1693-702
pubmed: 23669356
Adv Protein Chem. 1994;45:153-203
pubmed: 8154369
PLoS One. 2015 Oct 16;10(10):e0140097
pubmed: 26474480
Nature. 2013 May 30;497(7451):633-7
pubmed: 23665962
J Control Release. 2012 Apr 30;159(2):181-8
pubmed: 22285548
Mol Cell Proteomics. 2003 Oct;2(10):1096-103
pubmed: 12917320
Chembiochem. 2005 Jan;6(1):114-22
pubmed: 15549725
RSC Chem Biol. 2021 Dec 21;3(2):231-241
pubmed: 35360888
Cell Mol Life Sci. 2018 Apr;75(7):1227-1239
pubmed: 29119228
Nat Commun. 2021 Jun 17;12(1):3721
pubmed: 34140497
Adv Drug Deliv Rev. 2018 May;130:73-89
pubmed: 30012492
Adv Drug Deliv Rev. 2008 Mar 1;60(4-5):598-607
pubmed: 18045727