Optimized Simultaneous Axial Multifocal Imaging via Frequency Multiplexed Focusing.

Simultaneous axial multifocal imaging (SAMI) using a single acoustical transmission was developed to enhance the depth of field. This technique transmits a superposition of axial multifoci waveforms in a single transmission, thus increasing the frame rate. However, since all the waveforms are transmitted at a constant center frequency, there is a tradeoff between attenuation and lateral resolution when choosing a constant frequency for all the axial depths. In this work, we developed an optimized SAMI method by adding frequency dependence to each axial multifocus. By gradually increasing the frequency as a function of the focal depth, this method makes it possible to compensate for the gradually increasing F-number in order to achieve constant lateral resolution across the entire field of view. Alternatively, by gradually decreasing the axial multifoci frequencies as a function of depth, enhanced penetration depth and contrast are obtained. This method, termed frequency multiplexed SAMI (FM-SAMI), is described analytically and validated by resolution and contrast experiments performed on resolution targets, tissue-mimicking phantoms, and ex vivo biological samples. This is the first real-time implementation of a frequency multiplexing approach for axial multifoci imaging that facilitates high-quality imaging at an increased frame rate.