Probing the Effect of Rigidity on the Cellular Uptake of Core-Shell Nanoparticles: Stiffness Effects are Size Dependent.
RAFT emulsion polymerization
cellular uptake
elasticity
endocytosis
glass transition temperature
in vivo distribution
nanoparticles
rigidity
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
11
07
2022
received:
18
05
2022
pubmed:
20
8
2022
medline:
28
9
2022
entrez:
19
8
2022
Statut:
ppublish
Résumé
Nanoparticles are well established vectors for the delivery of a wide range of biomedically relevant cargoes. Numerous studies have investigated the impact of size, shape, charge, and surface functionality of nanoparticles on mammalian cellular uptake. Rigidity has been studied to a far lesser extent, and its effects are still unclear. Here, the importance of this property, and its interplay with particle size, is systematically explored using a library of core-shell spherical PEGylated nanoparticles synthesized by RAFT emulsion polymerization. Rigidity of these particles is controlled by altering the intrinsic glass transition temperature of their constituting polymers. Three polymeric core rigidities are tested: hard, medium, and soft using two particle sizes, 50 and 100 nm diameters. Cellular uptake studies indicate that softer particles are taken up faster and threefold more than harder nanoparticles with the larger 100 nm particles. In addition, the study indicates major differences in the cellular uptake pathway, with harder particles being internalized through clathrin- and caveolae-mediated endocytosis as well as macropinocytosis, while softer particles are taken up bycaveolae- and non-receptormediated endocytosis. However, 50 nm derivatives do not show any appreciable differences in uptake efficiency, suggesting that rigidity as a parameter in the biological regime may be size dependent.
Identifiants
pubmed: 35986441
doi: 10.1002/smll.202203070
doi:
Substances chimiques
Clathrin
0
Emulsions
0
Polymers
0
Polyethylene Glycols
3WJQ0SDW1A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2203070Subventions
Organisme : Cancer Research UK
ID : C53561/A19933
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBSRC ALERT14
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M01228X/1
Pays : United Kingdom
Informations de copyright
© 2022 The Authors. Small published by Wiley-VCH GmbH.
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