Tip60/Kat5 may be a novel candidate histone acetyltransferase for the regulation of liver iron localization via acetylation.
Expression
Hepcidin
Iron metabolism
Liver
Tip60
Journal
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine
ISSN: 1572-8773
Titre abrégé: Biometals
Pays: Netherlands
ID NLM: 9208478
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
received:
16
03
2022
accepted:
10
08
2022
pubmed:
21
8
2022
medline:
23
11
2022
entrez:
20
8
2022
Statut:
ppublish
Résumé
Hepcidin (HAMP), an iron regulatory hormone synthesized by liver hepatocytes, works together with ferritin (FTH) and ferroportin (FPN) in regulating the storage, transport, and utilization of iron in the cell. Epigenetic mechanisms, especially acetylation, also play an important role in the regulation of iron metabolism. However, a target protein has not been mentioned yet. With this preliminary study, we investigated the effect of histone acetyltransferase TIP60 on the expression of HAMP, FTH, and FPN. In addition, how the depletion of Tip60, which regulates the circadian system, affects the daily expression of Hamp was examined at six Zeitgeber time (ZT) points. For this purpose, liver-specific Tip60 knockout mice (mutant) were produced with tamoxifen-inducible Cre/lox recombination and an iron overload model in mice was generated. While HAMP and FTH expressions decreased, FPN expression increased in the mutant group. Interestingly, there was no change in the iron content. A significant increase was observed in the expressions of HAMP, FTH, and FPN and total liver iron content in the liver tissue of the iron overload group. Since intracellular iron concentration is involved in regulating the circadian clock, temporal expression of Hamp was investigated in control and mutant groups at six ZT points. In the control group, Hamp accumulated in a circadian manner with maximal and minimal levels reaching around ZT16 and ZT8, respectively. In the mutant group, there was a significant reduction in Hamp expression in the light phase ZT0 and ZT4 and in the dark phase ZT16. These data are the first findings demonstrating a possible relationship between Tip60 and iron metabolism.
Identifiants
pubmed: 35986817
doi: 10.1007/s10534-022-00435-z
pii: 10.1007/s10534-022-00435-z
doi:
Substances chimiques
Histone Acetyltransferases
EC 2.3.1.48
Iron
E1UOL152H7
Kat5 protein, mouse
EC 2.3.1.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1187-1197Subventions
Organisme : Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
ID : 114Z277
Organisme : Atatürk Üniversitesi
ID : PRJ2013/79
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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