BmCH25H, a vertebrate interferon-stimulated gene(ISG) homolog, inhibits BmNPV infection dependent on its hydroxylase activity in Bombyx mori.
Bombyx mori
Bombyx mori nucleopolyhedrovirus
Cholesterol 25-hydroxylase
Interferon-stimulated genes
Signal transducer and activator of transcription
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
revised:
10
07
2022
received:
29
04
2022
accepted:
26
07
2022
medline:
4
4
2023
pubmed:
22
8
2022
entrez:
21
8
2022
Statut:
ppublish
Résumé
Cholesterol-25-hydroxylase (CH25H) has been identified as an interferon-stimulated gene (ISG) in mammals that exerts its antiviral effects by catalyzing the conversion of cholesterol to 25-hydroxycholesterol (25HC). However, invertebrates lack an antiviral system homologous to vertebrate interferons (IFNs) because the genomes of invertebrates do not encode IFN-like cytokines. Nevertheless, CH25H is present in insect genomes and it therefore deserves further study of whether and by which mechanism it could exert an antiviral effect in invertebrates. In this study, the Bombyx mori CH25H (BmCH25H) gene, of which the encoded protein has high homology with other lepidopteran species, was identified and located on chromosome 9. Interestingly, we found that the expression of BmCH25H was significantly upregulated in B. mori nucleopolyhedrovirus (BmNPV) -infected BmN cells and silkworm (B. mori) larvae at the early infection stage. The inhibitory effect of BmCH25H on BmNPV replication was further demonstrated to depend on its catalytic residues to convert cholesterol to 25HC. More importantly, we demonstrated that during BmNPV infection, BmCH25H expression was increased through the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway, similar to the induction of ISGs following virus infection in vertebrates. This is the first report that CH25H has antiviral effects in insects; the study also elucidates the regulation of its expression and its mechanism of action.
Identifiants
pubmed: 35989418
doi: 10.1111/1744-7917.13102
doi:
Substances chimiques
Interferons
9008-11-1
Antiviral Agents
0
Cholesterol
97C5T2UQ7J
Mixed Function Oxygenases
EC 1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
321-337Informations de copyright
© 2022 Institute of Zoology, Chinese Academy of Sciences.
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