Development of a new TaqMan-based real-time RT-PCR assay for the specific detection of bovine kobuvirus.
3D gene
TaqMan probe
bovine kobuvirus
phylogenetic analysis
real-time RT-PCR
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
26
05
2022
accepted:
07
07
2022
entrez:
22
8
2022
pubmed:
23
8
2022
medline:
23
8
2022
Statut:
epublish
Résumé
Bovine kobuvirus (BKV) is a novel kobuvirus considered to be closely related to calf diarrhea and has become a worldwide epidemic. Currently, the BKV lacks an efficient and convenient detection method to assist the research on BKV prevalence. In this study, a new and specific TaqMan-based real-time RT-PCR for the detection of BKV was developed using the conserved region of the 3D gene. The assay was highly specific for BKV, without cross-amplification with other non-targeted pathogens. The limit of detection of this assay was 10
Identifiants
pubmed: 35990282
doi: 10.3389/fvets.2022.953599
pmc: PMC9386250
doi:
Types de publication
Journal Article
Langues
eng
Pagination
953599Informations de copyright
Copyright © 2022 Liu, Liu, Wang, Wang, Gao, Sun, Yuan, Li and Wang.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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