A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria.
IgG
alpha-Gal
bacteria
carbohydrate
monoclonal antibody
red meat allergy
xenotransplantation
α-Gal
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
01
06
2022
accepted:
18
07
2022
entrez:
22
8
2022
pubmed:
23
8
2022
medline:
24
8
2022
Statut:
epublish
Résumé
The alpha-Gal epitope (α-Gal) with the determining element galactose-α1,3-galactose can lead to clinically relevant allergic reactions and rejections in xenotransplantation. These immune reactions can develop because humans are devoid of this carbohydrate due to evolutionary loss of the enzyme α1,3-galactosyltransferase (GGTA1). In addition, up to 1% of human IgG antibodies are directed against α-Gal, but the stimulus for the induction of anti-α-Gal antibodies is still unclear. Commensal bacteria have been suggested as a causal factor for this induction as α-Gal binding tools such as lectins were found to stain cultivated bacteria isolated from the intestinal tract. Currently available tools for the detection of the definite α-Gal epitope, however, are cross-reactive, or have limited affinity and, hence, offer restricted possibilities for application. In this study, we describe a novel monoclonal IgG1 antibody (27H8) specific for the α-Gal epitope. The 27H8 antibody was generated by immunization of
Identifiants
pubmed: 35990627
doi: 10.3389/fimmu.2022.958952
pmc: PMC9391071
doi:
Substances chimiques
Antibodies, Monoclonal
0
Epitopes
0
Immunoglobulin G
0
Galactose
X2RN3Q8DNE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
958952Informations de copyright
Copyright © 2022 Kreft, Schepers, Hils, Swiontek, Flatley, Janowski, Mirzaei, Dittmar, Chakrapani, Desai, Eyerich, Deng, Niessing, Fischer, Feederle, Blank, Schmidt-Weber, Hilger, Biedermann and Ohnmacht.
Déclaration de conflit d'intérêts
MSD works as a consultant and an advisory board member at Theralution GmbH, Germany. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Cell. 2014 Dec 4;159(6):1277-89
pubmed: 25480293
Front Immunol. 2019 May 17;10:1056
pubmed: 31156631
Xenotransplantation. 1999 Nov;6(4):238-48
pubmed: 10704067
Science. 2002 Feb 8;295(5557):1089-92
pubmed: 11778012
J Biol Chem. 1991 Oct 25;266(30):20244-61
pubmed: 1939085
Front Mol Biosci. 2021 Nov 04;8:746883
pubmed: 34805272
J Allergy Clin Immunol. 2022 Feb;149(2):698-707.e3
pubmed: 34333031
Cell Host Microbe. 2021 Mar 10;29(3):347-361.e12
pubmed: 33497603
Sci Rep. 2016 Jun 29;6:29081
pubmed: 27353424
Microbiome. 2021 Jan 30;9(1):33
pubmed: 33516266
Elife. 2021 May 19;10:
pubmed: 34009123
Xenotransplantation. 2001 Feb;8(1):15-23
pubmed: 11208187
J Biol Chem. 1988 Nov 25;263(33):17755-62
pubmed: 2460463
Front Immunol. 2020 Oct 09;11:586924
pubmed: 33163001
J Biol Chem. 2011 Dec 16;286(50):43103-11
pubmed: 21990360
Nature. 1975 Aug 7;256(5517):495-7
pubmed: 1172191
J Allergy Clin Immunol. 2009 Oct;124(4):652-7
pubmed: 19815111
Front Immunol. 2022 Feb 17;13:807682
pubmed: 35250978
Br J Dermatol. 1974 May;90(5):525-30
pubmed: 4601016
Immunol Rev. 1994 Oct;141:31-58
pubmed: 7868156
Ticks Tick Borne Dis. 2020 Sep;11(5):101506
pubmed: 32723636
J Allergy Clin Immunol. 2019 Jan;143(1):182-189
pubmed: 30125663
J Biol Chem. 1995 Sep 15;270(37):21437-40
pubmed: 7545161
J Allergy Clin Immunol. 2014 Sep;134(3):755-759.e1
pubmed: 25171869
Clin Exp Allergy. 2014 Aug;44(8):1061-8
pubmed: 24750173
Allergy. 2016 May;71(5):711-9
pubmed: 26728983
JACS Au. 2021 Jul 12;1(8):1275-1287
pubmed: 34467365
J Immunol Methods. 1985 Apr 8;78(1):143-53
pubmed: 2580026
J Allergy Clin Immunol Pract. 2013 May-Jun;1(3):302-3
pubmed: 24565491
Appl Environ Microbiol. 2005 Dec;71(12):7995-8001
pubmed: 16332778
Front Immunol. 2020 Jan 13;10:3000
pubmed: 31998300
N Engl J Med. 2008 Mar 13;358(11):1109-17
pubmed: 18337601
Allergy. 2017 May;72(5):764-771
pubmed: 27775867
J Exp Med. 1925 Nov 30;42(6):863-72
pubmed: 19869096
J Biol Chem. 2002 Apr 26;277(17):14996-5001
pubmed: 11836253
J Allergy Clin Immunol. 2012 May;129(5):1334-1342.e1
pubmed: 22480538
J Immunol. 2007 Apr 1;178(7):4676-87
pubmed: 17372027
J Exp Med. 1984 Nov 1;160(5):1519-31
pubmed: 6491603
Science. 2003 Jan 17;299(5605):411-4
pubmed: 12493821
Allergy. 2012 May;67(5):699-704
pubmed: 22494361
J Allergy Clin Immunol. 2022 Aug;150(2):396-405.e11
pubmed: 35459547
J Clin Invest. 1992 Apr;89(4):1223-35
pubmed: 1556184
J Immunol Methods. 2011 Oct 28;373(1-2):26-35
pubmed: 21835180
Mol Immunol. 2000 Jun;37(8):455-66
pubmed: 11090880
Cell. 2016 Nov 17;167(5):1339-1353.e21
pubmed: 27863247
Transplantation. 1998 Apr 27;65(8):1129-32
pubmed: 9583877
Infect Immun. 1988 Jul;56(7):1730-7
pubmed: 3290105
Infect Immun. 2002 Nov;70(11):6215-22
pubmed: 12379700
Sci Rep. 2020 Mar 12;10(1):4611
pubmed: 32165720
Anal Chem. 2013 Jan 15;85(2):949-55
pubmed: 23215249
Exp Mol Med. 2017 Mar 10;49(3):e301
pubmed: 28280265
Allergy. 2021 Aug;76(8):2383-2394
pubmed: 33655520
Nat Biotechnol. 2002 Mar;20(3):251-5
pubmed: 11875425
Cancer Immunol Immunother. 2009 Oct;58(10):1545-56
pubmed: 19184002
J Allergy Clin Immunol. 2011 May;127(5):1286-93.e6
pubmed: 21453959
PLoS One. 2013;8(2):e55566
pubmed: 23390540