Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography.
Journal
Biomicrofluidics
ISSN: 1932-1058
Titre abrégé: Biomicrofluidics
Pays: United States
ID NLM: 101293825
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
01
10
2021
accepted:
25
07
2022
entrez:
22
8
2022
pubmed:
23
8
2022
medline:
23
8
2022
Statut:
epublish
Résumé
Speeding up and enhancing the performances of nucleic acid biosensing technologies have remained drivers for innovation. Here, we optimize a fluorimetry-based technology for DNA detection based on the concentration of linear targets paired with probes. The concentration module consists of a microfluidic channel with the shape of a funnel in which we monitor a viscoelastic flow and a counter-electrophoretic force. We report that the technology performs better with a target longer than 100 nucleotides (nt) and a probe shorter than 30 nt. We also prove that the control of the funnel geometry in 2.5D using grayscale lithography enhances sensitivity by 100-fold in comparison to chips obtained by conventional photolithography. With these optimized settings, we demonstrate a limit of detection of 4 fM in 30 s and a detection range of more than five decades. This technology hence provides an excellent balance between sensitivity and time to result.
Identifiants
pubmed: 35992636
doi: 10.1063/5.0073542
pii: 5.0073542
pmc: PMC9385222
doi:
Types de publication
Journal Article
Langues
eng
Pagination
044111Informations de copyright
© 2022 Author(s).
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