Isolation and characterization of uterine leukocytes collected using a uterine swab technique.

Female Humans Pregnancy Leukocytes, Mononuclear Placenta Uterus Killer Cells, Natural Immunophenotyping Leukocytes CD56 Antigen / metabolism Receptors, IgG / metabolism
PrimeFlow RNA cesarean section immunophenotyping maternal peripheral uterine leukocytes

Journal

American journal of reproductive immunology (New York, N.Y. : 1989)
ISSN: 1600-0897
Titre abrégé: Am J Reprod Immunol
Pays: Denmark
ID NLM: 8912860

Informations de publication

Date de publication:
11 2022
Historique:
revised: 07 07 2022
received: 02 01 2022
accepted: 15 08 2022
pubmed: 24 8 2022
medline: 4 11 2022
entrez: 23 8 2022
Statut: ppublish

Résumé

Leukocytes from the maternal-fetal interface are a valuable tool to study local changes in immune function during pregnancy; however, sampling can be challenging due to inadequate tissue availability and the invasive nature of placental bed biopsy. Here, we aim to purify and characterize leukocytes from paired peripheral and uterine blood samples to assess whether a less invasive method of uterine blood collection could yield a population of enriched uterine leukocytes suitable for ex vivo and in vitro analyses. Human peripheral blood mononuclear cells (PBMC) and uterine blood mononuclear cells (UBMC) expressed from surgical gauze post C-section were isolated, and immunophenotypic information was acquired by multi-parameter flow cytometry. PBMC and UBMC were stained for markers used to define T and B lymphocytes, macrophages, regulatory T (T Immunophenotyping revealed that over 95% of both live PBMC and UBMC consisted of CD45 Intrauterine leukocytes, especially CD16

Identifiants

DOI: 10.1111/aji.13614 PMID: 35997140 PMC: PMC9787928
pubmed: 35997140
doi: 10.1111/aji.13614
pmc: PMC9787928
doi:

Substances chimiques

CD56 Antigen 0
Receptors, IgG 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e13614

Subventions

Organisme : NICHD NIH HHS
ID : R21 HD083058
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD091429
Pays : United States

Informations de copyright

© 2022 The Authors. American Journal of Reproductive Immunology published by John Wiley & Sons Ltd.

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Auteurs

Parveen Parasar (P)

Department of Pathobiology & Diagnostic Investigation, Children's Hospital Boston, East Lansing, Michigan, USA.

Matthew Bernard (M)

Department of Pharmacology & Toxicology, Michigan State University, East Lansing, Michigan, USA.

Soo Hyun Ahn (SH)

Department of Pathobiology & Diagnostic Investigation, Children's Hospital Boston, East Lansing, Michigan, USA.

Sarika K Kshirsagar (SK)

Department of Pathobiology & Diagnostic Investigation, Children's Hospital Boston, East Lansing, Michigan, USA.

Sean L Nguyen (SL)

Cell and Molecular Biology Program, Michigan State University, East Lansing, Michigan, USA.
Institute for Integrative Toxicology, Michigan State University, East Lansing, Michigan, USA.

Geoffrey R Grzesiak (GR)

Department of Pathobiology & Diagnostic Investigation, Children's Hospital Boston, East Lansing, Michigan, USA.

Mathew Vettathu (M)

Department of Obstetrics & Gynecology, Sparrow Hospital, East Lansing, Michigan, USA.

Denny Martin (D)

Department of Obstetrics & Gynecology, Sparrow Hospital, East Lansing, Michigan, USA.

Margaret G Petroff (MG)

Department of Pathobiology & Diagnostic Investigation, Children's Hospital Boston, East Lansing, Michigan, USA.
Cell and Molecular Biology Program, Michigan State University, East Lansing, Michigan, USA.
Microbiology & Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Isolation and characterization of uterine...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Grossesse › Isolation and characterization of uterine...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Isolation and characterization of uterine...
questionsmedicales.fr › Structures de l'embryon › Placenta › Isolation and characterization of uterine...
questionsmedicales.fr › Appareil urogénital › Système génital › Système génital de la femme › Utérus › Isolation and characterization of uterine...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Cellules tueuses naturelles › Isolation and characterization of uterine...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Immunophénotypage › Isolation and characterization of uterine...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Isolation and characterization of uterine...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes de différenciation des lymphocytes T › Antigènes CD56 › Isolation and characterization of uterine...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteur Fc › Récepteurs du fragment Fc des IgG › Isolation and characterization of uterine...