Roles of RodZ and class A PBP1b in the assembly and regulation of the peripheral peptidoglycan elongasome in ovoid-shaped cells of Streptococcus pneumoniae D39.
class A PBP function and regulation
elongasome assembly
peptidoglycan synthesis
synthetic-viable genetic relationships
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
30
07
2022
received:
02
06
2022
accepted:
02
08
2022
pubmed:
25
8
2022
medline:
26
10
2022
entrez:
24
8
2022
Statut:
ppublish
Résumé
RodZ of rod-shaped bacteria functions to link MreB filaments to the Rod peptidoglycan (PG) synthase complex that moves circumferentially perpendicular to the long cell axis, creating hoop-like sidewall PG. Ovoid-shaped bacteria, such as Streptococcus pneumoniae (pneumococcus; Spn) that lack MreB, use a different modality for peripheral PG elongation that emanates from the midcell of dividing cells. Yet, S. pneumoniae encodes a RodZ homolog similar to RodZ in rod-shaped bacteria. We show here that the helix-turn-helix and transmembrane domains of RodZ(Spn) are essential for growth at 37°C. ΔrodZ mutations are suppressed by Δpbp1a, mpgA(Y488D), and ΔkhpA mutations that suppress ΔmreC, but not ΔcozE. Consistent with a role in PG elongation, RodZ(Spn) co-localizes with MreC and aPBP1a throughout the cell cycle and forms complexes and interacts with PG elongasome proteins and regulators. Depletion of RodZ(Spn) results in aberrantly shaped, non-growing cells and mislocalization of elongasome proteins MreC, PBP2b, and RodA. Moreover, Tn-seq reveals that RodZ(Spn), but not MreCD(Spn), displays a specific synthetic-viable genetic relationship with aPBP1b, whose function is unknown. We conclude that RodZ(Spn) acts as a scaffolding protein required for elongasome assembly and function and that aPBP1b, like aPBP1a, plays a role in elongasome regulation and possibly peripheral PG synthesis.
Identifiants
pubmed: 36001060
doi: 10.1111/mmi.14969
pmc: PMC9804626
doi:
Substances chimiques
Peptidoglycan
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
336-368Subventions
Organisme : NIAID NIH HHS
ID : F31 AI138430
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM141242
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131767
Pays : United States
Organisme : NIH HHS
ID : S10 OD024988
Pays : United States
Informations de copyright
© 2022 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.
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