Validation of Sputum Biomarker Immunoassays and Cytokine Expression Profiles in COPD.
airway colonisation
biomarker
chronic obstructive pulmonary disease
inflammatory endotype
sputum
validation
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
11 Aug 2022
11 Aug 2022
Historique:
received:
30
05
2022
revised:
02
08
2022
accepted:
08
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
27
8
2022
Statut:
epublish
Résumé
Immunoassays are commonly used to assess airway inflammation in sputum samples from chronic obstructive pulmonary disease (COPD) patients. However, assay performance and validation in this complex matrix is inconsistently reported. The aim of this study was to assess the suitability of various immunoassays for use with sputum samples, followed by use of validated immunoassays to evaluate biomarker levels in COPD patients. Assays were assessed for recombinant reference standard suitability, optimal sample dilution, standard recovery in the biological matrix and reproducibility. Validated assays were used to assess sputum supernatants in Cohort A (
Identifiants
pubmed: 36009496
pii: biomedicines10081949
doi: 10.3390/biomedicines10081949
pmc: PMC9405928
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : AstraZeneca (USA)
ID : Not Applicable
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