Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model.
immunocompromise
intrauterine growth restriction
microRNA
pig model
spleen
tsRNA
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
17 Aug 2022
17 Aug 2022
Historique:
received:
26
07
2022
revised:
14
08
2022
accepted:
16
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
27
8
2022
Statut:
epublish
Résumé
Intrauterine growth restriction (IUGR) is an important cause of newborn morbidity and mortality in mammals. Transfer RNA-derived small RNA (tsRNA) has become an emerging non-coding RNA in recent years. tsRNA and microRNAs (miRNAs) share similar mechanisms, which are involved in various biological processes. In this study, the pig was used as a model of IUGR, and the tsRNA and miRNA expression profile in the spleen was characterized by RNA sequencing. A total of 361 miRNAs and 620 tsRNAs were identified, of which 22 were differentially expressed miRNA (DEM) and 25 differentially expressed tsRNA (DET). tRF-5c were the primary tsRNA type making up more than 90%, and the most abundantly expressed tsRNAs are from tRNA-Gly-GCC. Functional enrichment analysis found that those DETs and DEMs have been implicated in the immune system process. Protein-protein interaction (PPI) network analysis revealed ssc-miR-370, ssc-miR-206, tiRNA-Ser-TGA-001 and tRF-Val-AAC-034 could be major regulators. TNF, TLR4, CD44, MAPK1 and STAT1 were predicted hub target genes. Those DETs and DEMs may regulate the T-cell receptor signaling pathway and Toll-like receptor signaling pathway to mediate the immunocompromise caused by IUGR. The results discussed in this article uncover the potential role of tsRNAs and miRNAs in IUGR porcine spleen.
Identifiants
pubmed: 36009692
pii: ani12162102
doi: 10.3390/ani12162102
pmc: PMC9404909
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Natural Science Foundation of China
ID : No.31902135; No.31972524
Organisme : Chongqing municipal key laboratory project
ID : No.21516
Organisme : Sichuan Science and Technology Program
ID : NO.2021YJ0265; NO.2021YFYZ0007; NO.2021ZDZX0008; NO.2020YFN0147; NO.scsztd-2022-08-09
Références
Anim Nutr. 2021 Sep;7(3):667-678
pubmed: 34430721
Chem Soc Rev. 2017 May 22;46(10):2844-2872
pubmed: 28352906
Nat Commun. 2017 Oct 24;8(1):1127
pubmed: 29066746
Ann Transl Med. 2020 Mar;8(5):233
pubmed: 32309380
Osteoarthritis Cartilage. 2020 Aug;28(8):1102-1110
pubmed: 32407895
Br J Nutr. 2015 Jul 14;114(1):53-62
pubmed: 26059215
J Hematol Oncol. 2022 May 7;15(1):53
pubmed: 35526007
Cell. 2015 May 7;161(4):790-802
pubmed: 25957686
Cell Prolif. 2021 Mar;54(3):e12977
pubmed: 33507586
Animals (Basel). 2021 May 27;11(6):
pubmed: 34071966
Anim Nutr. 2022 Jan 25;10:188-197
pubmed: 35785256
Cells. 2021 Apr 24;10(5):
pubmed: 33923344
Curr Pharm Biotechnol. 2016;17(10):848-55
pubmed: 27194361
Diagn Pathol. 2010 Apr 22;5:24
pubmed: 20412591
Am J Clin Nutr. 2007 Mar;85(3):845-52
pubmed: 17344508
Clin Obstet Gynecol. 2006 Jun;49(2):257-69
pubmed: 16721105
Mol Cancer Res. 2019 Jun;17(6):1403-1413
pubmed: 30872332
Eur J Clin Nutr. 2022 May;76(5):730-738
pubmed: 34611295
J Agric Food Chem. 2019 Mar 6;67(9):2709-2715
pubmed: 30701967
Oncol Lett. 2021 Jul;22(1):506
pubmed: 33986867
J Anim Sci. 2006 Sep;84(9):2316-37
pubmed: 16908634
Best Pract Res Clin Endocrinol Metab. 2019 Jun;33(3):101260
pubmed: 30709755
Nat Rev Drug Discov. 2017 Mar;16(3):203-222
pubmed: 28209991
Rev Bras Ginecol Obstet. 2020 May;42(5):289-296
pubmed: 32483809
Epigenomics. 2020 Feb;12(3):183-197
pubmed: 31845823
Am J Physiol Endocrinol Metab. 2020 Sep 1;319(3):E614-E628
pubmed: 32744097
Nature. 2017 Dec 7;552(7683):57-62
pubmed: 29186115
Mol Ther. 2020 Nov 4;28(11):2340-2357
pubmed: 32956625
Nat Immunol. 2015 Oct;16(10):1014-24
pubmed: 26382870
Biomolecules. 2019 Jul 11;9(7):
pubmed: 31336727
J Matern Fetal Neonatal Med. 2015 Feb;28(3):362-5
pubmed: 24853042
PLoS One. 2011;6(5):e20171
pubmed: 21637854
Oxid Med Cell Longev. 2022 Mar 19;2022:2644205
pubmed: 35345830
Dev Comp Immunol. 2021 Jan;114:103849
pubmed: 32888967
BMC Genomics. 2020 Oct 8;21(1):701
pubmed: 33032518
Methods. 2021 Mar;187:57-67
pubmed: 33045361
Mol Reprod Dev. 2011 Oct-Nov;78(10-11):879-91
pubmed: 21671302
Am J Physiol Regul Integr Comp Physiol. 2018 Dec 1;315(6):R1107-R1114
pubmed: 30207754
Neural Regen Res. 2020 Feb;15(2):212-221
pubmed: 31552886
Clin Med Insights Pediatr. 2016 Jul 14;10:67-83
pubmed: 27441006
Am J Obstet Gynecol. 2018 Feb;218(2S):S745-S761
pubmed: 29422210
Reprod Biol. 2014 Mar;14(1):51-60
pubmed: 24607255
Gut. 2022 Aug;71(8):1642-1655
pubmed: 34706869
Mol Metab. 2020 Dec;42:101083
pubmed: 32956848
J Exp Clin Cancer Res. 2021 Jul 5;40(1):222
pubmed: 34225773
J Dev Orig Health Dis. 2016 Feb;7(1):91-101
pubmed: 26442628
Am J Obstet Gynecol. 2018 Feb;218(2S):S869-S879
pubmed: 29422215
FASEB J. 2021 Aug;35(8):e21776
pubmed: 34324740
Evid Based Complement Alternat Med. 2015;2015:835263
pubmed: 26664411
J Anim Sci. 2021 Jul 1;99(7):
pubmed: 34107017
Trends Biochem Sci. 2016 Aug;41(8):679-689
pubmed: 27263052
Food Funct. 2021 Nov 15;12(22):11214-11228
pubmed: 34647565
Am J Physiol Gastrointest Liver Physiol. 2017 May 1;312(5):G434-G442
pubmed: 28280141
J Matern Fetal Neonatal Med. 2013 Mar;26(4):388-91
pubmed: 23035924
Food Funct. 2020 May 1;11(5):4456-4470
pubmed: 32374309
Front Immunol. 2021 Sep 01;12:706774
pubmed: 34539638
Gene. 2016 Apr 1;579(2):133-8
pubmed: 26743126
RNA Biol. 2020 Aug;17(8):1196-1213
pubmed: 32186461
FASEB J. 2019 Dec;33(12):13228-13240
pubmed: 31560576
Cancer Res. 1977 Sep;37(9):3362-6
pubmed: 884680
Nucleic Acids Res. 2016 Jan 4;44(D1):D184-9
pubmed: 26673694
Mucosal Immunol. 2021 Nov;14(6):1323-1334
pubmed: 34341503
Genome Med. 2021 Feb 9;13(1):20
pubmed: 33563322
Sci Rep. 2021 Aug 16;11(1):16569
pubmed: 34400677
Nucleic Acids Res. 2021 Sep 20;49(16):9077-9096
pubmed: 34417604
Cell Death Dis. 2022 May 18;13(5):471
pubmed: 35585048
Pediatr Res. 2009 Jan;65(1):45-50
pubmed: 18703996
Int J Biol Sci. 2018 Jun 22;14(9):1133-1141
pubmed: 29989099
Sci Transl Med. 2021 Nov 24;13(621):eabd5758
pubmed: 34818055
Cancer. 1979 Dec;44(6):2120-3
pubmed: 509391
Ultrasound Obstet Gynecol. 2009 Apr;33(4):421-6
pubmed: 19306477
Front Genet. 2018 Aug 15;9:291
pubmed: 30158951
Mol Cell. 2012 Nov 30;48(4):509-20
pubmed: 23084833