The Critical Role of Hypoxia in the Re-Differentiation of Human Articular Chondrocytes.
3D cell culture
cartilage
chondrocyte
hypoxia
re-differentiation
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
17 08 2022
17 08 2022
Historique:
received:
01
06
2022
revised:
01
08
2022
accepted:
07
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
30
8
2022
Statut:
epublish
Résumé
The preservation of the chondrogenic phenotype and hypoxia-related physiological microenvironment are major challenges in the 2D culture of primary human chondrocytes. To address this problem, we develop a 3D culture system generating scaffold-free spheroids from human chondrocytes. Our results highlight the chondrogenic potential of cultured human articular chondrocytes in a 3D system combined with hypoxia independently of the cartilage source. After 14 days of culture, we developed spheroids with homogenous diameter and shape from hyaline cartilage donors. Spheroids generated in hypoxia showed a significantly increased glycosaminoglycans synthesis and up-regulated the expression of
Identifiants
pubmed: 36010629
pii: cells11162553
doi: 10.3390/cells11162553
pmc: PMC9406483
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Cells. 2022 Apr 05;11(7):
pubmed: 35406794
J Clin Med. 2020 Aug 30;9(9):
pubmed: 32872610
Eur Cell Mater. 2011 Nov 09;22:275-90; discussion 290
pubmed: 22071698
Biomed Res. 2020;41(4):187-197
pubmed: 32801268
Stem Cell Res Ther. 2012 Mar 02;3(2):9
pubmed: 22385573
Arthritis Res Ther. 2005;7(3):R560-8
pubmed: 15899043
J Nanobiotechnology. 2020 Nov 9;18(1):163
pubmed: 33167997
Osteoarthritis Cartilage. 2012 Oct;20(10):1170-8
pubmed: 22796508
J Mater Chem B. 2019 Mar 14;7(10):1761-1772
pubmed: 32254918
Cartilage. 2020 Oct;11(4):521-531
pubmed: 30221989
Aging (Albany NY). 2021 Apr 4;13(8):11433-11454
pubmed: 33839696
Am J Sports Med. 2019 Aug;47(10):2348-2359
pubmed: 31265317
BMC Biotechnol. 2018 Mar 14;18(1):14
pubmed: 29540167
Arthritis Rheum. 2002 Feb;46(2):404-19
pubmed: 11840443
Lancet. 2015 Jul 25;386(9991):376-87
pubmed: 25748615
Arthritis Res Ther. 2009;11(5):R133
pubmed: 19723327
Rheum Dis Clin North Am. 2013 Feb;39(1):45-66
pubmed: 23312410
Int J Mol Sci. 2017 Sep 08;18(9):
pubmed: 28885597
Integr Biol (Camb). 2015 Mar;7(3):289-97
pubmed: 25614382
Osteoarthritis Cartilage. 2013 Apr;21(4):599-603
pubmed: 23376013
BMC Musculoskelet Disord. 2018 May 24;19(1):168
pubmed: 29793458
Ann Biomed Eng. 2020 Jan;48(1):298-311
pubmed: 31451988
Methods. 2008 May;45(1):2-9
pubmed: 18442700
Int J Mol Sci. 2020 Apr 13;21(8):
pubmed: 32294921
Cytotherapy. 2021 Jun;23(6):488-499
pubmed: 33092987
PLoS One. 2019 May 2;14(5):e0208291
pubmed: 31048887
J Bone Joint Surg Br. 2004 Mar;86(2):286-95
pubmed: 15046449
Osteoarthritis Cartilage. 2010 Oct;18(10):1345-54
pubmed: 20650328
Ann Rheum Dis. 2014 Sep;73(9):1659-64
pubmed: 23744977
Pharmaceutics. 2021 Jan 05;13(1):
pubmed: 33466397
Osteoarthritis Cartilage. 2011 Oct;19(10):1210-8
pubmed: 21816228
Int J Mol Sci. 2020 Feb 03;21(3):
pubmed: 32028724
Cartilage. 2018 Apr;9(2):202-211
pubmed: 29271232
Biomed Res Int. 2019 Nov 16;2019:5871698
pubmed: 31828107
Cells. 2019 Aug 19;8(8):
pubmed: 31430976
Cells. 2021 Sep 21;10(9):
pubmed: 34572145
Arthritis Res Ther. 2013 Aug 21;15(4):R92
pubmed: 23965235
Biomaterials. 2013 Jun;34(17):4266-73
pubmed: 23489925
Acta Orthop. 2011 Apr;82(2):234-40
pubmed: 21434761
Cells. 2019 Aug 24;8(9):
pubmed: 31450621
Cartilage. 2019 Oct;10(4):467-479
pubmed: 29671342
Biotechnol Prog. 2020 Jan;36(1):e2897
pubmed: 31461224
Cartilage. 2021 Dec;13(2_suppl):872S-884S
pubmed: 31540551
Biomaterials. 2019 Feb;192:569-578
pubmed: 30544046
J Mater Sci Mater Med. 2003 Aug;14(8):713-6
pubmed: 15348413
Bull Exp Biol Med. 2021 Feb;170(4):528-536
pubmed: 33725253
Sci Rep. 2019 Oct 10;9(1):14630
pubmed: 31601910
J Biomech. 2010 Sep 17;43(13):2516-23
pubmed: 20557888