Functional Characterization of the Venus Flytrap Domain of the Human TAS1R2 Sweet Taste Receptor.
G-protein-coupled receptor (GPCR)
class C GPCR
sugar
sweet taste
sweetener
taste
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Aug 2022
16 Aug 2022
Historique:
received:
27
06
2022
revised:
09
08
2022
accepted:
11
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
30
8
2022
Statut:
epublish
Résumé
The human sweet taste receptor is a heterodimeric receptor composed of two distinct G-protein-coupled receptors (GPCRs), TAS1R2 and TAS1R3. The TAS1R2 and TAS1R3 subunits are members of a small family of class C GPCRs whose members share the same architecture, comprising a Venus Flytrap (VFT) module linked to the seven transmembrane domains (TMDs) by a short cysteine-rich region (CRR). The VFT module of TAS1R2 contains the primary binding site for most of the sweet-tasting compounds, including natural sugars and artificial and natural sweeteners. However, cellular assays, molecular docking and site-directed mutagenesis studies have revealed that the VFT, CRR and TMD of TAS1R3 interact with some sweeteners, including the sweet-tasting protein brazzein. The aim of this study was to better understand the contribution of TAS1R2-VFT in the binding of sweet stimuli. To achieve this, we heterologously expressed human TAS1R2-VFT (hTAS1R2-VFT) in
Identifiants
pubmed: 36012481
pii: ijms23169216
doi: 10.3390/ijms23169216
pmc: PMC9409066
pii:
doi:
Substances chimiques
Ligands
0
Receptors, G-Protein-Coupled
0
Sweetening Agents
0
taste receptors, type 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Research Institute for Agriculture, Food and Environment
ID : XXX
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