The Effect of Necrotic Enteritis Challenge on Production Performance, Cecal Microbiome, and Cecal Tonsil Transcriptome in Broilers.
microbiome
necrotic enteritis
transcriptome
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
27 Jul 2022
27 Jul 2022
Historique:
received:
15
04
2022
revised:
14
07
2022
accepted:
20
07
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
27
8
2022
Statut:
epublish
Résumé
The objective of this study was to identify the effects of experimental necrotic enteritis (NE) infection on the production performance, gut microbiome, and cecal tonsil transcriptome in broiler birds. A total of 192 chicks were not-induced (control) or induced with NE. NE was induced by inoculating Eimeria maxima at 14 d of age and Clostridium perfringens at 19, 20, and 21 d of age. NE challenge increased (p < 0.01) NE lesion score at 7 days post-E.maxima infection (dpi), decreased (p < 0.01) average weight gain and increased (p < 0.01) mortality at 7 and 14 dpi. NE challenge increased (p < 0.05) gut permeability at 5, 6, and 7 dpi and increased ileal C. perfringens load at 5 dpi. NE challenge increased (p < 0.01) Eimeria oocyst shedding at 5, 6, 7, 8 and 14 dpi. NE challenge decreased (p < 0.05) the relative abundance of Lactobacillaceae and increased (p < 0.05) the relative abundance of Campylobacteriaceae, Comamonadaceae, and Ruminococcaceae at 6 dpi. NE challenge upregulated (p < 0.05) genes related to immune response and downregulated (p < 0.05) genes related to lipid metabolism at 6 dpi. It can be concluded that NE infection decreased beneficial bacteria and increased gut permeability.
Identifiants
pubmed: 36014961
pii: pathogens11080839
doi: 10.3390/pathogens11080839
pmc: PMC9414309
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : United States Department of Agriculture
ID : 2017-05035
Organisme : United States Department of Agriculture
ID : 58-6040-8-034
Organisme : Hatch grant
ID : 000
Références
Front Vet Sci. 2018 Aug 21;5:199
pubmed: 30186844
Vet Res. 2012 Oct 26;43:74
pubmed: 23101966
Science. 2018 Mar 9;359(6380):1161-1166
pubmed: 29420262
Arch Biochem Biophys. 1984 Jun;231(2):400-10
pubmed: 6329100
Nutr Metab Cardiovasc Dis. 2014 Jun;24(6):606-13
pubmed: 24602606
Poult Sci. 2021 Sep;100(9):101330
pubmed: 34280643
Poult Sci. 2007 Jul;86(7):1491-500
pubmed: 17575200
Poult Sci. 2021 Apr;100(4):100981
pubmed: 33647722
ISME J. 2013 Jul;7(7):1256-61
pubmed: 23677008
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Nucleic Acids Res. 2013 Jan 7;41(1):e1
pubmed: 22933715
Crit Rev Microbiol. 2001;27(2):75-131
pubmed: 11450855
Trends Biotechnol. 2015 Sep;33(9):496-503
pubmed: 26210164
Poult Sci. 2019 Jul 1;98(7):2800-2812
pubmed: 30877749
Front Microbiol. 2015 Nov 09;6:1266
pubmed: 26617600
J Biol Chem. 2002 Jan 4;277(1):148-54
pubmed: 11675382
Toxins (Basel). 2010 Jul;2(7):1595-611
pubmed: 22069652
Avian Dis. 2010 Sep;54(3):1058-65
pubmed: 20945788
FEMS Microbiol Lett. 1992 Jun 15;72(3):227-33
pubmed: 1354195
Oncotarget. 2018 Mar 27;9(23):16489-16500
pubmed: 29662661
Nucleic Acids Res. 2020 Jan 8;48(D1):D445-D453
pubmed: 31586394
Poult Sci. 2020 Apr;99(4):1847-1861
pubmed: 32241465
Nat Commun. 2020 Jan 24;11(1):483
pubmed: 31980623
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Avian Pathol. 2016 Jun;45(3):308-12
pubmed: 26950294
Mol Plant Pathol. 2012 Oct;13(8):805-15
pubmed: 22738439
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
Annu Rev Physiol. 2011;73:283-309
pubmed: 20936941
J Infect Dis. 1997 Jul;176(1):189-95
pubmed: 9207366
EMBO Rep. 2018 Mar;19(3):
pubmed: 29348145
Anim Nutr. 2015 Mar;1(1):1-11
pubmed: 29766984
Genome Biol Evol. 2011;3:1312-23
pubmed: 22002916
J Lab Physicians. 2019 Jan-Mar;11(1):87-90
pubmed: 30983809
Poult Sci. 2018 Jun 1;97(6):1899-1908
pubmed: 29538713
Nat Biotechnol. 2020 Jun;38(6):685-688
pubmed: 32483366
Vet Microbiol. 2012 Sep 14;159(1-2):155-62
pubmed: 22487456
Mol Biosyst. 2012 Aug;8(8):2097-105
pubmed: 22688384
Front Vet Sci. 2017 Apr 19;4:56
pubmed: 28470003
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
J Anim Sci Biotechnol. 2022 Feb 10;13(1):3
pubmed: 35139922
J Bioenerg Biomembr. 2007 Dec;39(5-6):465-71
pubmed: 17990087
Gut Microbes. 2014 Jan-Feb;5(1):108-19
pubmed: 24256702
J Anim Physiol Anim Nutr (Berl). 2020 Nov;104(6):1835-1850
pubmed: 32996177
Appl Environ Microbiol. 2007 Mar;73(6):1908-13
pubmed: 17259368
Poult Sci. 2005 Dec;84(12):1875-85
pubmed: 16479944
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
mBio. 2018 Sep 4;9(5):
pubmed: 30181248
BMC Microbiol. 2016 Aug 12;16(1):186
pubmed: 27520106
Front Microbiol. 2021 Aug 19;12:703693
pubmed: 34489892
J Bacteriol. 2002 Aug;184(15):4187-96
pubmed: 12107136
Avian Dis. 2010 Mar;54(1):33-40
pubmed: 20408396
Nat Rev Immunol. 2009 Nov;9(11):799-809
pubmed: 19855405
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Poult Sci. 2021 Jul;100(7):101190
pubmed: 34087701
Vet Microbiol. 2010 Jan 6;140(1-2):116-21
pubmed: 19647376
Vet Parasitol. 2010 Apr 19;169(1-2):1-7
pubmed: 20083358
Poult Sci. 2019 Jan 1;98(1):188-198
pubmed: 30239962
PLoS One. 2014 Dec 11;9(12):e114960
pubmed: 25504150
J Parasitol. 2018 Feb;104(1):18-22
pubmed: 28967300
Nutr Clin Pract. 2012 Apr;27(2):201-14
pubmed: 22367888
Vet Microbiol. 2014 Mar 14;169(3-4):188-97
pubmed: 24522272
FASEB J. 1991 Feb;5(2):156-63
pubmed: 1900793
Nat Rev Immunol. 2014 Oct;14(10):667-85
pubmed: 25234148
EBioMedicine. 2019 Jan;39:497-509
pubmed: 30415891
J Biol Chem. 1998 Jun 19;273(25):15559-64
pubmed: 9624146
Nat Biotechnol. 2019 Aug;37(8):852-857
pubmed: 31341288
Parasitol Res. 2012 Nov;111(5):1947-52
pubmed: 22828932
Vet Microbiol. 2015 Nov 18;180(3-4):299-303
pubmed: 26455806
Anal Biochem. 1987 Apr;162(1):156-9
pubmed: 2440339